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| Εξοπλισμός Μονάδας Οπτικής Μικροσκοπίας |
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:: Μονάδα :: Σεμινάρια :: Κανόνες Χρήσης :: Gallery ::
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:: Αίτηση Χρήσης ::
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| :: Workstation 1 :: Workstation 2 :: Workstation 3 :: Workstation 4 :: Plan of the unit :: |
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Workstation 1
Leica TCS-SP confocal Microscope
(http://www.confocal-microscopy.com/) |
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This is an older version of the Leica laser scanning confocals. However the measurement software is identical to the one on the Leica SP2 AOBS system.
• Microscope
DMR upright microscope
FITC and Rhodamine filter sets (for conventional epi-illmination).
• Objectives
10x 0.3 PH1 HC PL Fluotar dry
20x 0.7 PH2 HC PlanApo dry
40x 0.75-1.25 HCX PLApoCS oil
63x 0.6-1.32 HCX PLApo oil
• Lasers
Argon: 458, 488, 514 nm
He/Neon: 543, 633 nm
• Detectors
3 fluorscence PMT-s (spectral detection option)
1 transmission PMT
• Image acquisition and analysis software
Leica LCS |
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Workstation 2 –Image analysis
(Leica LCS, Image Pro, Photoshop) |
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Workstation 3 |
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Time lapse Olympus X81 Cell-R
The Olypmus Cell-R is a fully motorized epi-flurescence microscope equipped with a fast excitation filter wheel allowing for fast multicolor 3D imaging.
The 3D datasets can further be deconvolved with Deconvolution software (Cell R or Imaris). An on line Imaris software is installed in Workstation 3
• Stage
Olympus IX8S1F-ZDC (inverted), equipped for DIC and phase contrast imaging.
• Filters
3 filter sets (open page with filter combinations)
1) DAPI, FITC, TXRed (and their combination for fast imaging)
2) DAPI, FITC, C3, C5
3) CFP, YFP
• Objectives
10x 0.3 NA Ph1 UPlanFLN dry
20x 045 NA LUCPLanFLN dry
40x 0.6 NA LUCPLanFLN dry
60x 1.42 ΝΑ PlanApo oil
60x 1,2 NA UPLSApo water
100x 1.4 ΝΑ UPLSApo oil
• Temperature and CO2 chamber
• Camera
CCD ORCA/AG Hamamatsu |
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Workstation 4 - Image Analysis (Olympus Cell R, Imaris) |
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Facility Plan |
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