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a. Books Edited
“The function of neuroglia in Health and Disease - Dialogue between glia and neurons”, Series: Advances in Experimental Medicine and Biology. A scientific book of approximately 400 pages covering recent advances in glial cell biology and pathology, Plenum Press, 1999, edited by R. Matsas and M. Tsacopoulos.
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2008
Konstantakaki,
M, Tzartos, S.J., Poulas, K. and Eliopoulos, E. (2008). Μodel of the
extracellular domain of the human α7 nAChR based on the crystal
structure of the mouse α1 nAChR extracellular domain. J. Mol. Graph
Modelling. 26: 1333-7.
Bitzopoulou, K., ,Kostelidou, K.,
Poulas, K and Tzartos, S.J. (2008) Expression and characterization of
mutant forms of the extracellular domain of the human AChR
gamma-subunit with improved solubility and enhanced antigenicity.
Biochem. Biophys. Acta. 1784: 1226-33
Zisimopoulou, P.,
Lagoumintzis, G., Poulas, K. and Tzartos, S.J. (2008).
Antigen-specific apheresis of human anti-acetylcholine receptor
autoantibodies from myasthenia gravis patients’ sera using Escherichia
coli-expressed receptor domains. J. Neuroimmmunol. 200: 133-141.
Keefe,
D., Hess, D., Dosco, J., Tzartos, S., Powell, J., Lamsa. J., and
Josiah, S. (2008) A rapid, fluorescence based assay for detecting
antigenic modulation of the acetylcholine receptor on human cell lines.
Cytometry B Clin Cytom. Epub ahead of print.
Yi, H.J., Chae,
C.S., So, J.S., Tzartos, S.J. Souroujon MC, Fuchs, S., Im, SH (2008)
Suppression of experimental myasthenia gravis by a B-cell epitope-free
recombinant acetylcholine receptor. Mol. Immunol. 461:192-201.
Zouridakis
M, Zisimopoulou P, Eliopoulos E, Poulas K, and Tzartos SJ (2008) Design
and expression of human α7 nicotinic acetylcholine receptor
extracellular domain mutants with enhanced solubility and
ligand-binding properties. Bioch. Bioph. Acta. Epub ahead of print.
2007
Konstantakaki,
M., Tzartos, S.J., Poulas, K., and Eliopoulos, E. (2007) Molecular
modeling of the complex between Torpedo acetylcholine receptor and
anti-MIR Fab198. Biochem. Biophys. Res. Commun. 356:569-75.
Sideris,
S., Lagoumintzis, G., Kordas, G., Kostelidou, K., Sotiriadis, A,
Poulas, K. and Tzartos, S.J. (2007) Isolation and functional
characterization of anti-acetylcholine receptor subunit-specific
autoantibodies from myasthenic patients: receptor loss in cell culture.
J. Neuroimmunol. 189:111-7.
Zouridakis, M., Kostelidou, K.,
Sotiriadis, A., Stergiou, Ch. Eliopoulos, E., Poulas, K., and Tzartos,
S. (2007) Circular Dichroism Studies of Recombinant Extracellular
Domains of Human Muscle and Neuronal Nicotinic Acetylcholine Receptors
Provide an Insight into their structure. Intl. J. Biol. Macromol.
41:423-9.
Kostelidou, K., Trakas,N. and Tzartos, S.J. (2007)
Extracellular domains of the β, γ and ε subunits of the human
acetylcholine receptor as immunoadsorbents for myasthenic
autoantibodies: a combination of immunoadsorbents results in increased
efficiency. J. Neuroimmmunol. 190:44-52.
2006
Kostelidou,
K., Trakas,N., Zouridakis, M., Bitzopoulou, K., Sotiriadis,A., Gavra,
H., and Tzartos, S.J. (2006). Expression and characterization of
soluble forms of the extracellular domains of the β, γ and ε subunits
of the human muscle acetylcholine receptor. FEBS J. 273: 3557-3568
Fostieri,
E., Tzartos, S.J., Berrih-Aknin, S., Beeson, D. and Mamalaki A. (2005)
Isolation of potent human Fab fragments against a novel highly
immunogenic region on human muscle acetylcholine receptor, which
protect the receptor from myasthenic autoantibodies. Eur. J. Immunol.
35:632-643.
2000- 2005
Protopapadakis,
E., Kokla, A., Tzartos, S.J. and Mamalaki, A (2005) Isolation and
characterization of human anti-acetylcholine receptor monoclonal
antibodies from transgenic mice expressing human immunoglobulin
loci. Eur. J. Immunol. 35:1960-68.
Guyon, S., Christadoss, P.,
Lepanse, R, Guyon, T, DePoea Baets, M., Wakkach, A., Bidault, J.,
Tzartos, S., and Berrih-Aknin, S. (2005) Effects of cytokines on AChR
expression. Implications for myasthenia gravis. J. Immunol. 174:
5941-49.
Psaridi-Linardaki, L., Mamalaki, A., Trakas, N. and
Tzartos, S.J. (2005) Specific immunoadsorption of the autoantibodies
from myasthenic patients using the extracellular domain of the human
muscle acetylcholine receptor α-subunit. Development of an
antigen-specific therapeutic strategy. J. Neuroimmunol. 159:183-91.
Avramopoulou,
V., Mamalaki, A., Tzartos, S.J. (2004) Soluble, oligomeric and
ligand-binding extracellular domain of human alpha7 acetylcholine
receptor expressed in yeast. Replacement of the hydrophobic
cys-loop by the hydrophilic loop of ACh-binding protein enhances
protein solubility. J. Biol. Chem. 279: 38287-93.
Marinou,
M. and Tzartos, S.J. (2003). Identification of regions involved in the
binding of α-Bungarotoxin to the human α7 neuronal nicotinic
acetylcholine receptor using synthetic peptides. Biochem. J. 372,
543-554.
Phan-Chan-Du, A., Hemmerlin, C., Krikorian, D.,
Sakarellos-Daitsiotis, M., Tsikaris, V., Sakarellos, C., Marinou.
M.,Thureau, A., Cung, M.T. and Tzartos, S.J. (2003). Solution
conformation of the antibody-bound tyrosine phosphorylation site of
the nicotinic acetylcholine -subunit in its phosphorylated and
nonphosphorylated states.receptor Biochemistry, 42:7371-7380.
Campos,
E.C., Schiavi, C., Bolognesi, A., Bellusci, C., Lubelli, C., Duca, A.,
Polito, L., Vismara, S., Poulas, K., Tzartos, S.I., and Stirpe, F.
(2002). Selective lesions of rabbit extraocular muscles injected with
the anti-AChR immunotoxin saporin-mAb 73. Curr. Eye Res. 24: 58-65.
Psaridi-Linardaki,
L.,Mamalaki, A., Remoundos, M. and Tzartos, S.J. (2002). Expression of
soluble ligand- and antibody-binding extracellular subunit in yeast
Pichiadomain of human muscle acetylcholine receptor α- pastoris. Role
of glycosylation in α-bungarotoxin binding. J. Biol. Chem. 277: 26980-6.
Μetaxas,
A., Tzartos, S., Liakopoulou-Kyriakide, M. (2002). The production of
anti-hexapeptide antibodies which recognize the S7, L6 and L13
ribosomal proteins of Escherichia coli. J Pept Sci. 8:118-24.
Tataridis,
D., Kolocouris, A., Fytas, G., Kolocouris, N., Foscolos, G.B., Poulas,
K. and Tzartos, S.J. (2002).Mounting the nicotinic pharmacophoric
structural elements in an homoadamantane scaffold: synthesis, molecular
modeling and binding affinities to α7 nicotinic acetylcholine
receptors. IL Farmaco. 57:979-984.
Poulas, K. and Tzartos, S.J (2001). The gender gap in autoimmune disease. Letter to Lancet, 357, 234.
Poulas,
K., Eliopoulos, E., Vatzaki, E., Navaza, J., Kontou, M., Oikonomakos,
N., Acharya, K.R., and Tzartos, S. J. (2001). Crystal structure of
Fab198, an efficient protector of acetylcholine receptor against
myasthenogenic antibodies. Eur. J. Biochem. 268: 3685-3693.
Poulas,
K., Tsibri, E., Papanastasiou, D., Tsouloufis, T., Marinou, M.,
Tsantili, P., Papapetropoulos, T., and Tzartos, S.J. (2001).
Epidemiology of seropositive myasthenia gravis in Greece. J. Neurol.
Neurosur. Psych. 71:352-6.
Theodorou, V.,Tsikaris, V.,
Sakarellos-Daitsiotis, M, Avramopoulou, V., Kostelidou, K., Tzartos,
S.J. and Sakarellos, C. (2001). Design, synthesis and
conformational study of biologically active photolabeled analogues of
the Main Immunogenic Region of the acetylcholine receptor. Biopolymers.
56: 37-46.
Trakas, N. and Tzartos, S.J. (2001) Conjugation of
acetylcholine receptor-protecting Fab fragments with polyethylene
glycol results in a prolonged half-life in the circulation and reduced
immunogenicity. J. Neuroimmunol. 120:42-49.
Skok, M.,
Lykhmus, E., Bobrovnik, S., Tzartos, S.I., Tsouloufis, T., Vanderesse,
R. , Coutrot, F., Cung, M.T., Marraud, M., Krikorian, D., and
Sakarellos-Daitsiotis, M. (2001). Structure of epitopes recognized by
the antibodies to alpha (181-192) peptides of neuronal nicotinic
acetylcholine receptors: extrapolation to the structure of
acetylcholine-binding domain. J. Neuroimmunol. 121: 59-66.
Kleinjung,
J., Petit, M-C., Orlewski, P., Mamalaki, A., Tzartos, S.J., Tsikaris,
V., Sakarellos-Daitsiotis, M., Sakarellos, C., Marraud, M. and Cung.
M.-T. (2000). The 3D structure of the complex between an Fv
antibody fragment and an analogue of the main immunogenic region of the
acetylcholine receptor: A combined 2D-NMR, homology and molecular
modeling approach. Biopolymers. 53: 113-128
Sevin-Landais, A.,
Rigler, P., Tzartos, S., Hucho, F., Hovius, R. and Vogel, H. (2000).
Immobilisation of the nicotinic acetylcholine receptor in tethered
lipid membranes. Biophys. Chem. 85:141-52.
Poulas, K.,
Tsouloufis, Th., and Tzartos S.J. (2000) Treatment of passively
transferred experimental autoimmune myasthenia gravis using papain.
Clin. Expl. Immunol. 120:363-368.
Tsouloufis, T., Mamalaki, A.,
Remoundos, M. and Tzartos, S.J. (2000). Reconstitution of
conformationally-dependent epitopes on the N-terminal extracellular
domain of the human muscle acetylcholine receptor α-subunit
expressed in E. coli. Implications for Myasthenia Gravis therapeutic
approaches. Int. Immunol. 12: 1255-65.
Papanastasiou, D.,
Poulas, K., Kokla, A and Tzartos, S. J. (2000). Prevention of passively
transferred experimental autoimmune myasthenia gravis by Fab
fragments of monoclonal antibodies directed against the main
immunogenic region of the acetylcholine receptor. J. Neuroimmunol.
104:124-132.
Kontou, M., Leonidas, D., Vatzaki, E.H., Tsantili,
P., Mamalaki, A., Oikonomakos, N.G., Acharya, K.R, and Tzartos, S. J.
(2000).The crystal structure of the Fab fragment of a rat monoclonal
antibody against the main immunogenic region of the human muscle
acetylcholine receptor. Eur. J. Biochem. 267:2389-2397
Michalet,
S., Teixeira, F., Gilquin, B., Mourier, G., Servent, S., Drevet, P.,
Binder, P., Tzartos, S., Ménez, A., and Kessler, P. (2000). Relative
spatial position of a snake neurotoxin and the reduced disulfide bond
αCys192-Cys193) at the αγ interface of the nicotinic acetylcholine
receptor. J. Biol. Chem. 275: 25608-25615.
Sieb, J.P., Kraner,
S., Schrank, B., Reitter, B., Goebel, H.H., Tzartos, S. J. and
Steinlein, O.K. (2000). Severe congenital myasthenic syndrome due to
homozygosity of the 1293insG ε-AChR subunit mutation. Ann. Neurol.
48:379-83
Fostieri, E., Beeson, D. and Tzartos, S.J. (2000). The
conformation of the main immunogenic region on the α-subunit of muscle
acetylcholine receptor is affected by neighboring receptor subunits.
FEBS Lett. 481: 127-130.
Poulas, K., Tsibri, E., Papanastasiou,
D., Tsouloufis, T., Marinou, M., Tsantili, P., Papapetropoulos, T., and
Tzartos, S.J. (2000). Equal male and female incidence of myasthenia
gravis. Neurology, 54, 1202-1203.
1990-1999
Nenninger,
R., Schultz, A., Helmreich, M., Wilisch, A., Vandekerckhove, B., Hunig,
T., Schalke, B., Tzartos, S.J., Kahlbacher, H., Muller-Hermelink, H.
and Marx, A. (1998). Abnormal thymocyte development - Generation of
autoaggressive T-cells in mixed and cortical thymomas. Lab. Invest.
78:743-753.
Tsantili, P., Tzartos, S.J. and Mamalaki, A. (1999)
High affinity scFv antibody fragments protecting the human nicotinic
acetylcholine receptor. J. Neuroimmunol. 94: 15-27.
Papanastasiou,
D., Mamalaki, A., Eliopoulos, E., Poulas, K., Liolitsas Ch. and
Tzartos, S.J. (1999). Construction and characterization of a humanized
single chain Fv antibody fragment against the main immunogenic region
of the acetylcholine receptor. J. Neuroimmunol. 94: 182-195.
Tzartos,
S.J. and Remoundos, M. (1999). Detection of antibodies directed against
the cytoplasmic region of the human acetylcholine receptor in sera from
myasthenia gravis patients. Clin. Expl. Immunol. 116: 146-152.
Skok,
M.V., Voitenko, L. P., Voitenko, S. V., Lykhmus, E. Yu., Kalashnik, E.
N., Litvin, T. I., Tzartos S. J. and Skok, V. I. (1999). Alpha subunit
composition of nicotinic acetylcholine receptors in the rat autonomic
ganglia neurons as determined with subunit-specific anti-α(181-192)
peptide antibodies. Neuroscience. .93: 1436-1447.
Wilisch, A.,
Gutsche, S., Hoffacker, V., Schultz, A., Tzartos, S., Nix, W., Schalke,
B., Schneider, C., Muller- Hermelink, H. K. and Marx A. (1999).
Association of acetylcholine receptor α-subunit expression in mixed
thymoma with myasthenia gravis. Neurology. 52: 1460-1466.
Schultz,
A., Hoffacker, V.,Wilisch, A., Nix, W., Schalke, B., Nix, W., Tzartos,
S., Muller-Hermelink, H. K. and Marx A. (1999). Neurofilament is an
autoantigenic determinant in myasthenia gravis. Ann. Neurology. 46:
167-75.
Martin-Ruiz, C.M., Court, J.A., Molnar, Ε., Lee, Μ.,
Gotti, C., Mamalaki, A., Tsouloufis, T., Tzartos, S., Ballard, C.,
Perry, R.H. and Perry, E.K. (1999) Alpha 4 but not alpha 3 and 7
nicotinic acetylcholine receptor subunits are lost from the temporal
cortex in Alzheimer's disease. J. Neurochem. 73: 1635-1640.
Jacobson,
L., Beeson, D., Tzartos, S. and Vincent, A. (1999). Monoclonal
antibodies raised against human acetylcholine receptor bind to all five
subunits of the fetal isoform. J. Neuroimmunol. 98: 112-120.
Wakkach,
A., Poea, S., Chastre, E., Gespach, C., Lecerf, F., De la Porte, S.,
Tzartos, S., Coulombe, A., and Berrih-Aknin, S. (1999). Establishment
of a thymic myoid cell line: phenotypic and functional characteristics.
Am. J. Pathol. 155:1229-1240
Sieb, J.P., Dorfler, P., Tzartos,
S., Wewer, U., Ruegg, M.,Meyer, D., Baumann, I., Lindemuth, R.,
Jakschik, J. and Ries, F. (1998). Congenital myasthenic syndromes in
two kinships with endplate acetylcholine receptor and utrophin
deficiency. Neurology, 50: 54-61.
De la Porte, S., Chaubourt,
E., Fabre, F., Poulas, K., Chapron, J., Eymard, B., Tzartos, S.J., and
Koenig, J. (1998). Accumulation of acetylcholine receptors is a
necessary condition for normal accumulation of acetylcholinesterase
during in vivo neuromuscular synaptogenesis. Eur. J. Neurosci., 10:
1631-1643.
Barchan, D., Asher, O., Tzartos, S.J., Fuchs, S. and
Souroujon, M. (1998). Modulation of the anti-acetylcholine receptor
and experimental autoimmune myasthenia gravis by recombinant fragments
of the acetylcholine receptor. Eur. J. Immunol. 28:616-624.
Gattenloehner,
S., Vincent, A., Leuschner, I., Tzartos, S., Kirchner, T.,
Muller-Hermelink, H.-K., Marx, A. (1998) Investigation of the
acetylcholine receptor in rhabdomyosarcomas as compared with other
childhood tumors. Am. J. Pathol. 152: 437-444.
Guyon, T.,
Wakkach, A., Klingel-Schmitt, I., Levasseur, P., Beeson, D., Asher, O.,
Tzartos, S.J. and Berrih-Aknin, S. (1998). Regulation of acetylcholine
receptor gene expression in human myasthenia gravis muscles. Evidence
for a compensatory mechanism triggered by receptor loss. J. Clin.
Invest. 102: 249-263.
Orlewski, P., Marraud, M., Cung, M.T,
Tsikaris, V., Sakarellos-Daitsiotis, M., Sakarellos, K., Vatzaki, E.
and Tzartos, S.J. (1996). Compared structures of the free AChR MIR
decapeptide and the antibody-bound [A76]MIR analogue. A molecular
dynamics simulation from 2D-NMR data. Biopolymers (Peptide Science).
40: 419-432.
Gotti, C., Balestra, B., Mantegazza, R., Tzartos,
S., Moretti, M. & Clementi, F. (1997) Detection of antibody
subpopulations in myasthenia gravis using a non radioactive new enzyme
immunoassay. Muscle & Nerve, 20: 800-808.
Loutrari, H.,
Kokla, A., N. Trakas and Tzartos, S.J. (1997). Expression of
human-Torpedo hybrid acetylcholine receptors for analyzing the
antigenic specificities in myasthenic antisera. Clin. Expl. Immunol.
109, 538-546.
Andre, F., Marraud, M., Tsouloufis, T., Tzartos,
S.J., Boussard, G. (1997) Triphosgene: An efficient carbonylating agent
for liquid and solid-phase aza-peptide synthesis. Application to the
synthesis of two aza-analogues of the AChR MIR decapeptide. J. Pep.
Science . 3: 429-441
Voltz, R., Kamm, C., Padberg, F., Malotka,
J., Kerschensteiner, M., Spuler, S., Tzartos, S., and Dornmair, K.
(1997) Highly purified oligo-His tagged human recombinant alpha(1)-AChR
is immunogenic in vivo and suitable for T cell stimulation in vitro in
experimental and human myasthenia gravis. J.Neuroimmunol.
80:131-136.
Bufler, J., Kahlert, S., Tzartos, S.J., Toyka, C.
and Franke, C. (1996). Activation and blockage of nicotinic
channels by antibodies directed against the binding site of the
acetylcholine receptor. J. Physiol. 492: 107-114
Kontou, M.,
Vatzaki, E.H., Kokla, A., Acharya, K.R., Oikonomakos, N.G. and Tzartos,
S.J. (1996). Characterisation, crystallisation and preliminary X-ray
diffraction analysis of a Fab fragment of a rat monoclonal antibody
with very high affinity for the human muscle acetylcholine receptor.
FEBS Lett. 389: 195-198.
Wakkach, A., Guyon, T., Bruand, C.,
Tzartos, S., Cohen-Kaminsky, S. and Berrih-Aknin, S. (1996) Expression
of acetylcholine receptor genes in human thymic epithelial cells.
Implication for myasthenia gravis. J. Immunol. 157: 3752-3760.
Pappas,
I.S., Sophianos, D., Tzartos, S.J. and Tsiftsoglou, A.S. (1996).
Expression of memory, differentiation and repression of c-myc and p53
genes in human RD/TE-671 cells induced by a uveido-derivative of
pyridine (UDP-4). Cell Growth and Differentiation. 7: 797-809.
Tsikaris,
V., Sakarellos, C., Sakarellos-Daitsiotis, M., Orlewski, P., Marraud,
M. Cung, M.T., Vatzaki, E., and Tzartos, S.J. (1996). Construction
and application of a new class of sequential oligopeptide carriers for
multiple anchoring of antigenic peptides. Application to the AChR main
immunogenic region. Intern. J. Biol. Macromol. 19: 195-205.
Tzartos,
S.J., Kouvatsou, R. and Tzartos E. (1995) Monoclonal antibodies as
site-specific probes for the acetylcholine receptor δ-subunit tyrosine
and serine phosphorylation sites. Eur. J. Biochem. 228, 463-472.
Mapouras,
D., Philippou, G., Charalabous, S., Tzartos, S.J. Balafas, A.,
Souvatzoglou, A. and Lymberi, P. (1995). Antibodies to
acetylcholinesterase cross-reacting with thyroglobulin in myasthenia
gravis and graves disease. Clin. Exp. Immunol 100: 336-343.
Tzartos,
S.J., Tzartos, E., and Tzartos, J.S. (1995) Monoclonal antibodies
against the acetylcholine receptor γ-subunit as site specific probes
for receptor tyrosine phosphorylation. FEBS Letters. 363: 195-198.
Mamalaki,
A., Boutou, E., Hurel, C., Patsavoudi, E., Tzartos, S.J. and Matsas, R.
(1995). The BM88 antigen, a novel neuron-specific molecule,
promotes the differentiation of mouse neuroblastoma cells. J. Biol.
Chem. 270: 14201-14208.
Asher, O., Kues, W.A., Witzemann,
V.,Tzartos, S.J., Fuchs, S. and Souroujon, M.C. (1993) Increased gene
expression of acetylcholine receptor and myogenic factors in passively
transferred experimental autoimmune myasthenia gravis. J. Immunol. 151,
6442-6450.
Schmutz, M., Kling, D., Tzartos, S.J. and Brisson, A.
(1994). A mixed ELISA-immunoelectron microscopy approach for
studying epitope topology of membrane proteins: application to the
acetylcholine receptor. J. Histochem. Cytochem. 42, 315-327 (1994).
Tzartos,
S.J., Valcana, C., Kouvatsou, R., and Kokla, A. (1993) The tyrosine
phosphorylation site of the acetylcholine receptor β-subunit is
located in a highly immunogenic epitope implicated in channel
function. Antibody-probes for β subunit phosphorylation and function.
EMBO J. 12, 5141-5149.
Papadouli, I., Sakarellos, C.,and
Tzartos, S.T. (1993). High resolution epitope mapping and fine
antigenic characterization of the main immunogenic region of the
acetylcholine receptor. Improving the binding activity of synthetic
analogues of the region. Eur. J. Biochem. 211, 227-234.
Eleftheriou,
C.S., Trakas, N., Kokla, A. and Tzartos, S.J. (1993). A group of three
fibroblast secreted polypeptides suppressed by cellular ageing and
interferon-γ. Bioch. Bioph. Acta. 1180, 304-312.
Detsikas, E.,
Tsikaris, V., Sakarellos-Daitsiotis, M., Sakarellos, C., Cung, M.T.,
Marraud, M., Vatzaki, E. and Tzartos, S.J. (1993). Cyclic lactam
analogues containing the main immunogenic region of Torpedo
acetylcholine receptor. Peptide Research 6, 17-23.
Tsikaris, V.,
Detsikas, E., Sakarellos-Daitsiotis, M., Sakarellos, C., Vatzaki, E.,
Tzartos, S.J., Marraud, M. and Cung, M.T., (1993). Conformational
requirements for molecular recognition of ACh receptor MIR analogues by
monoclonal anti-MIR antibody: A 2D-NMR and molecular dynamics approach.
Biopolymers. 33, 1123-1134.
Mamalaki, A., Trakas, N., and
Tzartos, S.J. (1993). Bacterial expression of a single-chain Fv
fragment which efficiently protects the acetylcholine receptor against
antigenic modulation caused by myasthenic antibodies. Eur. J.
Immunol. 23, 1839-1845.
Melms, A., Weissert, R., Klinkert,
W.E.F., Schalke, B.C.G., Tzartos, S. and Wekerle, H. (1993). Specific
immune complexes augment in vitro acetylcholine receptor-specific
T-cell proliferation. Neurology 43, 583-588.
Vatzaki, E. H.,
Acharya, K.R., Oikonomakos, N. G. and Tzartos, S.J. (1993).
Crystallization and preliminary crystallographic study of an Fab
fragment of a pathogenic rat monoclonal antibody against the nicotinic
acetylcholine receptor. Protein Science. 2: 1770-1772.
Loutrari,
H., Tzartos, S.J. and Claudio, T. (1992). Use of Torpedo-mouse hybrid
acetylcholine receptors reveals immunodominance of the α-subunit in
myasthenia gravis antisera. Eur. J. Immunol. 22, 2949-2956.
Loutrari,
H., Kokla, A. and Tzartos, S.J. (1992). Passive transfer of
experimental myasthenia gravis via antigenic modulation of
acetylcholine receptor. Eur. J. Immunol. 22, 2449-2452.
Zhang,
Y., and Tzartos, S.J. (1992). B-T lymphocyte interactions in
experimental autoimmune myasthenia gravis. Autoantibody mediated
up-regulation of the response of AChR-specific T-lymphocytes.
Immunology 77, 571-576.
Marx, A., Osborn, M., Tzartos, S.,
Geuder, K.I., Schalke, B., Nix, W., Kirchner, T., and Muller-Hermelink,
H.K. (1992). A striational muscle antigen and myasthenia
gravis-associated thymomas share an acetylcholine receptor epitope.
Develop. Immunol. 2, 77-84.
Cung, M.T., Demange, P., Marraud,
M., Tsikaris, V., Sakarellos, C., Papadouli, I., Kokla, A., Tzartos,
S.J. (1991). Two-dimentional 1H-NMR study of antigen-antibody
interactions: binding of synthetic decapeptides to an
anti-acetylcholine receptor monoclonal antibody. Biopolymers. 31,
769-776.
Verschuuren, J.J.G.M., Graus Y.M.F., Bos N.A., Tzartos
S.J., Van Breda Vriesman P.J.C., and De Baets M.H. (1991). Paratope and
framework related idiotopes on acetylcholine receptor antibodies. J.
Immunol. 146, 941-948.
Verschuuren, J.J.G.M., Graus Y.M.F., Van
Breda Vriesman P.J.C., Tzartos S.J., and De Baets M.H. (1991). In vivo
effects of neonatal administration of anti-idiotype antibodies on
experimental autoimmune myasthenia gravis Autoimmunity. 10:173-179.
Tzartos,
S. J., Loutrari, H., Tang, F., Kokla, A., Walgrave, S., Milius, R. P.
and Conti-Tronconi, B. (1990). Main immunogenic region of Torpedo
electroplax and human muscle acetylcholine receptor. Localization and
micro-heterogeneity reveiled by the use of synthetic peptides. J.
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Plinkert, P.K., Gitter, A.H., Zimmermann,
U., Kirchner, T., Tzartos, S. and Zenner, H.P. (1990). Visualization
and functional testing of acetylcholine receptor-like molecules in
cochlear outer hair cells. Hearing Res. 44, 25-34.
Marx, A., O'
Connor, R., Geuder, K.I., Hoppe, F., Schalke, B., Tzartos, S., Kalies,
I., Kirchner, T., and Muller-Hermelink, H. K. (1990). Characterization
of a protein with an acetylcholine receptor epitope from myasthenia
gravis-associated thymomas. Lab. Invest.. 62, 279-286.
Tzartos,
S.J., Efthimiadis, A., Morel, E. and Bach, J.F. (1990) Neonatal
myasthenia gravis: antigenic specificities of antibodies in sera from
mothers and their infants. Clin. Exp. Immunol. 80, 376-380.
Papadouli,
I., Potamianos, S., Hadjidakis, I., Bairaktari, H., Tsikaris, V.,
Sakarellos, C., Cung, M.T., Marraud, M. and Tzartos, S.J. (1990).
Antigenic role of single residues within the main immunogenic region of
the nicotinic acetylcholine receptor. Biochem. J. 269, 239-245.
Tzartos,
S.J and Remoundos, M.S. (1990). Fine localization of the major
α-bungarotoxin binding site to residues α189-195 of the Torpedo
acetylcholine receptor. Residues 189, 190 and 195 are indispensable for
binding. J. Biol. Chem. 265, 21462-21467.
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2000-2008
Tzartos,
S.J., Bitzopoulou, K., Gavra, I., Kordas, G., Jacobson, L., Kostelidou,
K., Lagoumintzis, G., Lazos, O., Poulas, K., Sideris, S., Sotiriadis,
A., Trakas, N.,and
Zisimopoulou, P. (2008) Antigen-specific
apheresis of pathogenic autoantibodies from myasthenia gravis sera.
Ann. N.Y Acad. Sci., 1132:291-9.
Kostelidou, K., Sideri, A.,
Lazaridis, K., Fostieri, E., and Tzartos, S. (2008). Emerging therapies
for the treatment of autoimmune myasthenia gravis. Βόκ: Clinical
Applications of Immunomics, Immunomics Series. Ed. Andras Falus.
Springer. pp. 171-205.
Zisimopoulou, P., Lagoumintzis, G.,
Kostelidou, K., Bitzopoulou, K., Kordas, G., Trakas, N., Poulas, K.
and Tzartos, S.J. (2008) Towards antigen-specific apheresis of
pathogenic autoantibodies as a further step in the treatment of
myasthenia gravis by plasmapheresis. J. Neuroimmmunol. 201, 95-103.
Zouridakis
M, Zisimopoulou P, Poulas K, and Tzartos SJ (2008) Recent advances in
understanding the structure of nicotinic acetylcholine receptors. IUBMB
Life. Epub ahead of print
Kalamida, D., Poulas, K.,
Avramopoulou, V, Fostieri, E., Lagoumintzis, G., Lazaridis, K., Sideri
A., Zouridakis M. and Tzartos, S.J. (2007). Muscle and neuronal
nicotinic acetylcholine receptors: structure, function and
pathogenicity. FEBS J. 274, 3799-845
1990-1999
Fostieri,
E., Kostelidou, K, Poulas K., and Tzartos S.J. Recent advances in the
understanding and therapy of myasthenia gravis. Future Neurology, 1,
799-817.
Tzartos, S.J., Barkas, T., Cung, M. T., Mamalaki, A.,
Marraud, M., Orlewski, P., Papanastasiou, D., Sakarellos, C.,
Sakarellos Daitsiotis, M., Tsantili, P., and Tsikaris, V. (1998).
Anatomy of the antigenic structure of a large membrane autoantigen, the
muscle-type nicotinic acetylcholine receptor. Immunol. Rev. 163, 89-120.
Tzartos,
S.J. (1996). Epitope mapping by antibody competition. Methodology and
evaluation of the validity of the technique. In: Epitope Mapping
Protocols (ed. G.E. Morris). Series: Methods in Molecular Biology, vol.
66, pp. 55-66.
Mamalaki, A. and Tzartos, S.J. (1994). Nicotinic
acetylcholine receptor: structure, function and main immunogenic
region. Adv. Neuroimmunol. 4, 339-354.
Tzartos, S.J., Cung,
M.T., Demange, P., Loutrari, H., Mamalaki, A., Marraud, M., Papadouli,
I., Sakarellos, C. and Tsikaris, V. (1992) The main immunogenic region
(MIR) of the nicotinic acetylcholine receptor and the anti-MIR
antibodies. Molec. Neurobiol. 5, 1-29.
Tzartos, S.J., Barkas,
T., Cung I. Kordossi, A. Loutrari, E., Marraud, M., Papadouli, I.,
Sakarellos, C., Sophianos, D. and V. Tsikaris (1991). The main
immunogenic region of the acetylcholine receptor. Structure and role in
myasthenia gravis. Autoimmunity. 8, 259-270.
Tzartos, S.J.
(1991). Studing the acetylcholine receptor with monoclonal antibodies.
In "Current Aspects of the Neurosciences" (ed. N.N. Osborne) vol. 3.
The Macmillan Press, London. pp195-226.
Eleftheriou, C.S.,
Trakas, N. and Tzartos, S.J. (1991). Cellular aging related proteins
secreted by human fibroblasts. Mutation Research, 256, 127-138.
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