2009
Keefe, D., Hess, D., Dosco, J., Tzartos, S., Powell, J., Lamsa. J.,
and Josiah, S. (2009) A rapid, fluorescence based assay for detecting
antigenic modulation of the acetylcholine receptor on human cell lines.
Cytometry B Clin Cytom. 76(3):206-12.
Zouridakis M, Zisimopoulou P, Eliopoulos E, Poulas K, and Tzartos SJ
(2009) Design and expression of human α7 nicotinic acetylcholine
receptor extracellular domain mutants with enhanced solubility and
ligand-binding properties. Bioch. Bioph. Acta. 1794: 355-66.
Tsiamalos, P., Kordas, G., Kokla, A., Poulas, K., and Tzartos, S.J.
(2009). Epidemiological and immunological profile of MuSK myasthenia
gravis in Greece. Eur. J. Neurol. 16: 925-30.
Lykhmus, O , Koval, L., Pavlovych, S., Zouridakis, M., Zisimopoulou,
P., Tzartos, S., Tsetlin, V., Volpina, O., Cloëz-Tayarani, I.,
Komisarenko, S., and Skok, M. (2009) Functional effects of antibodies
against non-neuronal nicotinic acetylcholine receptors. Immunol. Lett.
128(1):68-73.
Gattenlohner, S, Jörißen, H., Huhn, M., Vincent, A., Beeson, D.,
Tzartos, S., Mamalaki, A., Etschmann, B., Müller-Hermelink, H.K.,
Koscielniak, E., Barth S.and Marx, A. (2009). A human recombinant
autoantibody-based immunotoxin specific for the fetal acetylcholine
receptor inhibits rhabdomyosarcoma growth in vitro and in a murine
transplantation model. J. Biomed. Biotech. In press.
Keefe, D., Parng , C., Lundberg, D., Ray, S., Martineau-Bosc, J.,
Leng, C., Tzartos, S., Powell , J., Concino, M., Michael Heartlein, M.,
Lamsa. J., and Josiah, S. (2010). In vitro characterization of an
acetylcholine receptor-transferrin fusion protein for the treatment of
Myasthenia Gravis. Autoimmunity, In press.
2008
Konstantakaki, M, Tzartos, S.J., Poulas, K. and Eliopoulos, E.
(2008). Μodel of the extracellular domain of the human α7 nAChR based
on the crystal structure of the mouse α1 nAChR extracellular domain. J.
Mol. Graph Modelling. 26: 1333-7.
Bitzopoulou, K., ,Kostelidou, K., Poulas, K and Tzartos, S.J. (2008)
Expression and characterization of mutant forms of the extracellular
domain of the human AChR gamma-subunit with improved solubility and
enhanced antigenicity. Biochem. Biophys. Acta. 1784: 1226-33.
Zisimopoulou, P., Lagoumintzis, G., Poulas, K. and Tzartos, S.J.
(2008). Antigen-specific apheresis of human anti-acetylcholine receptor
autoantibodies from myasthenia gravis patients’ sera using Escherichia
coli-expressed receptor domains. J. Neuroimmmunol. 200: 133-141.
Yi, H.J., Chae, C.S., So, J.S., Tzartos, S.J. Souroujon MC, Fuchs,
S., Im, SH (2008) Suppression of experimental myasthenia gravis by a
B-cell epitope-free recombinant acetylcholine receptor. Mol. Immunol.
461:192-201.
2007
Konstantakaki, M., Tzartos, S.J., Poulas, K., and Eliopoulos, E.
(2007) Molecular modeling of the complex between Torpedo acetylcholine
receptor and anti-MIR Fab198. Biochem. Biophys. Res. Commun.
356:569-75.
Sideris, S., Lagoumintzis, G., Kordas, G., Kostelidou, K.,
Sotiriadis, A, Poulas, K. and Tzartos, S.J. (2007) Isolation and
functional characterization of anti-acetylcholine receptor
subunit-specific autoantibodies from myasthenic patients: receptor loss
in cell culture. J. Neuroimmunol. 189:111-7.
Zouridakis, M., Kostelidou, K., Sotiriadis, A., Stergiou, Ch.
Eliopoulos, E., Poulas, K., and Tzartos, S. (2007) Circular Dichroism
Studies of Recombinant Extracellular Domains of Human Muscle and
Neuronal Nicotinic Acetylcholine Receptors Provide an Insight into
their structure. Intl. J. Biol. Macromol. 41:423-9.
Kostelidou, K., Trakas,N. and Tzartos, S.J. (2007) Extracellular
domains of the β, γ and ε subunits of the human acetylcholine receptor
as immunoadsorbents for myasthenic autoantibodies: a combination of
immunoadsorbents results in increased efficiency. J. Neuroimmmunol.
190:44-52.
2006
Kostelidou, K., Trakas,N., Zouridakis, M., Bitzopoulou, K.,
Sotiriadis,A., Gavra, H., and Tzartos, S.J. (2006). Expression and
characterization of soluble forms of the extracellular domains of the
β, γ and ε subunits of the human muscle acetylcholine receptor. FEBS J.
273: 3557-3568
Fostieri, E., Tzartos, S.J., Berrih-Aknin, S., Beeson, D. and
Mamalaki A. (2005) Isolation of potent human Fab fragments against a
novel highly immunogenic region on human muscle acetylcholine receptor,
which protect the receptor from myasthenic autoantibodies. Eur. J.
Immunol. 35:632-643.
2000- 2005
Protopapadakis, E., Kokla, A., Tzartos, S.J. and Mamalaki, A (2005)
Isolation and characterization of human anti-acetylcholine receptor
monoclonal antibodies from transgenic mice expressing human
immunoglobulin loci. Eur. J. Immunol. 35:1960-68.
Guyon, S., Christadoss, P., Lepanse, R, Guyon, T, DePoea Baets, M.,
Wakkach, A., Bidault, J., Tzartos, S., and Berrih-Aknin, S. (2005)
Effects of cytokines on AChR expression. Implications for myasthenia
gravis. J. Immunol. 174: 5941-49.
Psaridi-Linardaki, L., Mamalaki, A., Trakas, N. and Tzartos, S.J.
(2005) Specific immunoadsorption of the autoantibodies from myasthenic
patients using the extracellular domain of the human muscle
acetylcholine receptor α-subunit. Development of an antigen-specific
therapeutic strategy. J. Neuroimmunol. 159:183-91.
Avramopoulou, V., Mamalaki, A., Tzartos, S.J. (2004) Soluble,
oligomeric and ligand-binding extracellular domain of human alpha7
acetylcholine receptor expressed in yeast. Replacement of the
hydrophobic cys-loop by the hydrophilic loop of ACh-binding protein
enhances protein solubility. J. Biol. Chem. 279: 38287-93.
Marinou, M. and Tzartos, S.J. (2003). Identification of regions
involved in the binding of α-Bungarotoxin to the human α7 neuronal
nicotinic acetylcholine receptor using synthetic peptides. Biochem. J.
372, 543-554.
Phan-Chan-Du, A., Hemmerlin, C., Krikorian, D.,
Sakarellos-Daitsiotis, M., Tsikaris, V., Sakarellos, C., Marinou.
M.,Thureau, A., Cung, M.T. and Tzartos, S.J. (2003). Solution
conformation of the antibody-bound tyrosine phosphorylation site of
the nicotinic acetylcholine -subunit in its phosphorylated and
nonphosphorylated states.receptor Biochemistry, 42:7371-7380.
Campos, E.C., Schiavi, C., Bolognesi, A., Bellusci, C., Lubelli, C.,
Duca, A., Polito, L., Vismara, S., Poulas, K., Tzartos, S.I., and
Stirpe, F. (2002). Selective lesions of rabbit extraocular muscles
injected with the anti-AChR immunotoxin saporin-mAb 73. Curr. Eye
Res. 24: 58-65.
Psaridi-Linardaki, L.,Mamalaki, A., Remoundos, M. and Tzartos, S.J.
(2002). Expression of soluble ligand- and antibody-binding
extracellular subunit in yeast Pichiadomain of human muscle
acetylcholine receptor α- pastoris. Role of glycosylation in
α-bungarotoxin binding. J. Biol. Chem. 277: 26980-6.
Μetaxas, A., Tzartos, S., Liakopoulou-Kyriakide, M. (2002). The
production of anti-hexapeptide antibodies which recognize the S7, L6
and L13 ribosomal proteins of Escherichia coli. J Pept Sci. 8:118-24.
Tataridis, D., Kolocouris, A., Fytas, G., Kolocouris, N., Foscolos,
G.B., Poulas, K. and Tzartos, S.J. (2002).Mounting the nicotinic
pharmacophoric structural elements in an homoadamantane scaffold:
synthesis, molecular modeling and binding affinities to α7 nicotinic
acetylcholine receptors. IL Farmaco. 57:979-984.
Poulas, K. and Tzartos, S.J (2001). The gender gap in autoimmune disease. Letter to Lancet, 357, 234.
Poulas, K., Eliopoulos, E., Vatzaki, E., Navaza, J., Kontou, M.,
Oikonomakos, N., Acharya, K.R., and Tzartos, S. J. (2001). Crystal
structure of Fab198, an efficient protector of acetylcholine receptor
against myasthenogenic antibodies. Eur. J. Biochem. 268: 3685-3693.
Poulas, K., Tsibri, E., Papanastasiou, D., Tsouloufis, T., Marinou,
M., Tsantili, P., Papapetropoulos, T., and Tzartos, S.J. (2001).
Epidemiology of seropositive myasthenia gravis in Greece. J. Neurol.
Neurosur. Psych. 71:352-6.
Theodorou, V.,Tsikaris, V., Sakarellos-Daitsiotis, M, Avramopoulou,
V., Kostelidou, K., Tzartos, S.J. and Sakarellos, C. (2001).
Design, synthesis and conformational study of biologically active
photolabeled analogues of the Main Immunogenic Region of the
acetylcholine receptor. Biopolymers. 56: 37-46.
Trakas, N. and Tzartos, S.J. (2001) Conjugation of acetylcholine
receptor-protecting Fab fragments with polyethylene glycol results
in a prolonged half-life in the circulation and reduced immunogenicity.
J. Neuroimmunol. 120:42-49.
Skok, M., Lykhmus, E., Bobrovnik, S., Tzartos, S.I., Tsouloufis, T.,
Vanderesse, R. , Coutrot, F., Cung, M.T., Marraud, M., Krikorian, D.,
and Sakarellos-Daitsiotis, M. (2001). Structure of epitopes recognized
by the antibodies to alpha (181-192) peptides of neuronal nicotinic
acetylcholine receptors: extrapolation to the structure of
acetylcholine-binding domain. J. Neuroimmunol. 121: 59-66.
Kleinjung, J., Petit, M-C., Orlewski, P., Mamalaki, A., Tzartos,
S.J., Tsikaris, V., Sakarellos-Daitsiotis, M., Sakarellos, C., Marraud,
M. and Cung. M.-T. (2000). The 3D structure of the complex between an
Fv antibody fragment and an analogue of the main immunogenic region
of the acetylcholine receptor: A combined 2D-NMR, homology and
molecular modeling approach. Biopolymers. 53: 113-128
Sevin-Landais, A., Rigler, P., Tzartos, S., Hucho, F., Hovius, R.
and Vogel, H. (2000). Immobilisation of the nicotinic acetylcholine
receptor in tethered lipid membranes. Biophys. Chem. 85:141-52.
Poulas, K., Tsouloufis, Th., and Tzartos S.J. (2000) Treatment of
passively transferred experimental autoimmune myasthenia gravis using
papain. Clin. Expl. Immunol. 120:363-368.
Tsouloufis, T., Mamalaki, A., Remoundos, M. and Tzartos, S.J.
(2000). Reconstitution of conformationally-dependent epitopes on the
N-terminal extracellular domain of the human muscle acetylcholine
receptor α-subunit expressed in E. coli. Implications for Myasthenia
Gravis therapeutic approaches. Int. Immunol. 12: 1255-65.
Papanastasiou, D., Poulas, K., Kokla, A and Tzartos, S. J. (2000).
Prevention of passively transferred experimental autoimmune
myasthenia gravis by Fab fragments of monoclonal antibodies directed
against the main immunogenic region of the acetylcholine receptor. J.
Neuroimmunol. 104:124-132.
Kontou, M., Leonidas, D., Vatzaki, E.H., Tsantili, P., Mamalaki, A.,
Oikonomakos, N.G., Acharya, K.R, and Tzartos, S. J. (2000).The crystal
structure of the Fab fragment of a rat monoclonal antibody against the
main immunogenic region of the human muscle acetylcholine receptor.
Eur. J. Biochem. 267:2389-2397
Michalet, S., Teixeira, F., Gilquin, B., Mourier, G., Servent, S.,
Drevet, P., Binder, P., Tzartos, S., Ménez, A., and Kessler, P. (2000).
Relative spatial position of a snake neurotoxin and the reduced
disulfide bond αCys192-Cys193) at the αγ interface of the nicotinic
acetylcholine receptor. J. Biol. Chem. 275: 25608-25615.
Sieb, J.P., Kraner, S., Schrank, B., Reitter, B., Goebel, H.H.,
Tzartos, S. J. and Steinlein, O.K. (2000). Severe congenital myasthenic
syndrome due to homozygosity of the 1293insG ε-AChR subunit mutation.
Ann. Neurol. 48:379-83
Fostieri, E., Beeson, D. and Tzartos, S.J. (2000). The conformation
of the main immunogenic region on the α-subunit of muscle acetylcholine
receptor is affected by neighboring receptor subunits. FEBS Lett. 481:
127-130.
Poulas, K., Tsibri, E., Papanastasiou, D., Tsouloufis, T., Marinou,
M., Tsantili, P., Papapetropoulos, T., and Tzartos, S.J. (2000). Equal
male and female incidence of myasthenia gravis. Neurology, 54,
1202-1203.
1990-1999
Nenninger, R., Schultz, A., Helmreich, M., Wilisch, A.,
Vandekerckhove, B., Hunig, T., Schalke, B., Tzartos, S.J., Kahlbacher,
H., Muller-Hermelink, H. and Marx, A. (1998). Abnormal thymocyte
development - Generation of autoaggressive T-cells in mixed and
cortical thymomas. Lab. Invest. 78:743-753.
Tsantili, P., Tzartos, S.J. and Mamalaki, A. (1999) High affinity
scFv antibody fragments protecting the human nicotinic acetylcholine
receptor. J. Neuroimmunol. 94: 15-27.
Papanastasiou, D., Mamalaki, A., Eliopoulos, E., Poulas, K.,
Liolitsas Ch. and Tzartos, S.J. (1999). Construction and
characterization of a humanized single chain Fv antibody fragment
against the main immunogenic region of the acetylcholine receptor. J.
Neuroimmunol. 94: 182-195.
Tzartos, S.J. and Remoundos, M. (1999). Detection of antibodies
directed against the cytoplasmic region of the human acetylcholine
receptor in sera from myasthenia gravis patients. Clin. Expl. Immunol.
116: 146-152.
Skok, M.V., Voitenko, L. P., Voitenko, S. V., Lykhmus, E. Yu.,
Kalashnik, E. N., Litvin, T. I., Tzartos S. J. and Skok, V. I. (1999).
Alpha subunit composition of nicotinic acetylcholine receptors in the
rat autonomic ganglia neurons as determined with subunit-specific
anti-α(181-192) peptide antibodies. Neuroscience. .93: 1436-1447.
Wilisch, A., Gutsche, S., Hoffacker, V., Schultz, A., Tzartos, S.,
Nix, W., Schalke, B., Schneider, C., Muller- Hermelink, H. K. and
Marx A. (1999). Association of acetylcholine receptor α-subunit
expression in mixed thymoma with myasthenia gravis. Neurology. 52:
1460-1466.
Schultz, A., Hoffacker, V.,Wilisch, A., Nix, W., Schalke, B., Nix,
W., Tzartos, S., Muller-Hermelink, H. K. and Marx A. (1999).
Neurofilament is an autoantigenic determinant in myasthenia gravis.
Ann. Neurology. 46: 167-75.
Martin-Ruiz, C.M., Court, J.A., Molnar, Ε., Lee, Μ., Gotti, C.,
Mamalaki, A., Tsouloufis, T., Tzartos, S., Ballard, C., Perry, R.H.
and Perry, E.K. (1999) Alpha 4 but not alpha 3 and 7 nicotinic
acetylcholine receptor subunits are lost from the temporal cortex in
Alzheimer's disease. J. Neurochem. 73: 1635-1640.
Jacobson, L., Beeson, D., Tzartos, S. and Vincent, A. (1999).
Monoclonal antibodies raised against human acetylcholine receptor bind
to all five subunits of the fetal isoform. J. Neuroimmunol. 98:
112-120.
Wakkach, A., Poea, S., Chastre, E., Gespach, C., Lecerf, F., De la
Porte, S., Tzartos, S., Coulombe, A., and Berrih-Aknin, S. (1999).
Establishment of a thymic myoid cell line: phenotypic and functional
characteristics. Am. J. Pathol. 155:1229-1240
Sieb, J.P., Dorfler, P., Tzartos, S., Wewer, U., Ruegg, M.,Meyer,
D., Baumann, I., Lindemuth, R., Jakschik, J. and Ries, F. (1998).
Congenital myasthenic syndromes in two kinships with endplate
acetylcholine receptor and utrophin deficiency. Neurology, 50: 54-61.
De la Porte, S., Chaubourt, E., Fabre, F., Poulas, K., Chapron, J.,
Eymard, B., Tzartos, S.J., and Koenig, J. (1998). Accumulation of
acetylcholine receptors is a necessary condition for normal
accumulation of acetylcholinesterase during in vivo neuromuscular
synaptogenesis. Eur. J. Neurosci., 10: 1631-1643.
Barchan, D., Asher, O., Tzartos, S.J., Fuchs, S. and Souroujon, M.
(1998). Modulation of the anti-acetylcholine receptor and
experimental autoimmune myasthenia gravis by recombinant fragments of
the acetylcholine receptor. Eur. J. Immunol. 28:616-624.
Gattenloehner, S., Vincent, A., Leuschner, I., Tzartos, S.,
Kirchner, T., Muller-Hermelink, H.-K., Marx, A. (1998) Investigation of
the acetylcholine receptor in rhabdomyosarcomas as compared with other
childhood tumors. Am. J. Pathol. 152: 437-444.
Guyon, T., Wakkach, A., Klingel-Schmitt, I., Levasseur, P., Beeson,
D., Asher, O., Tzartos, S.J. and Berrih-Aknin, S. (1998). Regulation of
acetylcholine receptor gene expression in human myasthenia gravis
muscles. Evidence for a compensatory mechanism triggered by receptor
loss. J. Clin. Invest. 102: 249-263.
Orlewski, P., Marraud, M., Cung, M.T, Tsikaris, V.,
Sakarellos-Daitsiotis, M., Sakarellos, K., Vatzaki, E. and Tzartos,
S.J. (1996). Compared structures of the free AChR MIR decapeptide and
the antibody-bound [A76]MIR analogue. A molecular dynamics simulation
from 2D-NMR data. Biopolymers (Peptide Science). 40: 419-432.
Gotti, C., Balestra, B., Mantegazza, R., Tzartos, S., Moretti, M.
& Clementi, F. (1997) Detection of antibody subpopulations in
myasthenia gravis using a non radioactive new enzyme immunoassay.
Muscle & Nerve, 20: 800-808.
Loutrari, H., Kokla, A., N. Trakas and Tzartos, S.J. (1997).
Expression of human-Torpedo hybrid acetylcholine receptors for
analyzing the antigenic specificities in myasthenic antisera. Clin.
Expl. Immunol. 109, 538-546.
Andre, F., Marraud, M., Tsouloufis, T., Tzartos, S.J., Boussard, G.
(1997) Triphosgene: An efficient carbonylating agent for liquid and
solid-phase aza-peptide synthesis. Application to the synthesis of two
aza-analogues of the AChR MIR decapeptide. J. Pep. Science . 3:
429-441
Voltz, R., Kamm, C., Padberg, F., Malotka, J., Kerschensteiner, M.,
Spuler, S., Tzartos, S., and Dornmair, K. (1997) Highly purified
oligo-His tagged human recombinant alpha(1)-AChR is immunogenic in vivo
and suitable for T cell stimulation in vitro in experimental and human
myasthenia gravis. J.Neuroimmunol. 80:131-136.
Bufler, J., Kahlert, S., Tzartos, S.J., Toyka, C. and Franke, C.
(1996). Activation and blockage of nicotinic channels by antibodies
directed against the binding site of the acetylcholine receptor. J.
Physiol. 492: 107-114
Kontou, M., Vatzaki, E.H., Kokla, A., Acharya, K.R., Oikonomakos,
N.G. and Tzartos, S.J. (1996). Characterisation, crystallisation and
preliminary X-ray diffraction analysis of a Fab fragment of a rat
monoclonal antibody with very high affinity for the human muscle
acetylcholine receptor. FEBS Lett. 389: 195-198.
Wakkach, A., Guyon, T., Bruand, C., Tzartos, S., Cohen-Kaminsky, S.
and Berrih-Aknin, S. (1996) Expression of acetylcholine receptor
genes in human thymic epithelial cells. Implication for myasthenia
gravis. J. Immunol. 157: 3752-3760.
Pappas, I.S., Sophianos, D., Tzartos, S.J. and Tsiftsoglou, A.S.
(1996). Expression of memory, differentiation and repression of c-myc
and p53 genes in human RD/TE-671 cells induced by a uveido-derivative
of pyridine (UDP-4). Cell Growth and Differentiation. 7: 797-809.
Tsikaris, V., Sakarellos, C., Sakarellos-Daitsiotis, M., Orlewski,
P., Marraud, M. Cung, M.T., Vatzaki, E., and Tzartos, S.J. (1996).
Construction and application of a new class of sequential oligopeptide
carriers for multiple anchoring of antigenic peptides. Application to
the AChR main immunogenic region. Intern. J. Biol. Macromol. 19:
195-205.
Tzartos, S.J., Kouvatsou, R. and Tzartos E. (1995) Monoclonal
antibodies as site-specific probes for the acetylcholine receptor
δ-subunit tyrosine and serine phosphorylation sites. Eur. J. Biochem.
228, 463-472.
Mapouras, D., Philippou, G., Charalabous, S., Tzartos, S.J. Balafas,
A., Souvatzoglou, A. and Lymberi, P. (1995). Antibodies to
acetylcholinesterase cross-reacting with thyroglobulin in myasthenia
gravis and graves disease. Clin. Exp. Immunol 100: 336-343.
Tzartos, S.J., Tzartos, E., and Tzartos, J.S. (1995) Monoclonal
antibodies against the acetylcholine receptor γ-subunit as site
specific probes for receptor tyrosine phosphorylation. FEBS Letters.
363: 195-198.
Mamalaki, A., Boutou, E., Hurel, C., Patsavoudi, E., Tzartos, S.J.
and Matsas, R. (1995). The BM88 antigen, a novel neuron-specific
molecule, promotes the differentiation of mouse neuroblastoma cells. J.
Biol. Chem. 270: 14201-14208.
Asher, O., Kues, W.A., Witzemann, V.,Tzartos, S.J., Fuchs, S. and
Souroujon, M.C. (1993) Increased gene expression of acetylcholine
receptor and myogenic factors in passively transferred experimental
autoimmune myasthenia gravis. J. Immunol. 151, 6442-6450.
Schmutz, M., Kling, D., Tzartos, S.J. and Brisson, A. (1994). A
mixed ELISA-immunoelectron microscopy approach for studying epitope
topology of membrane proteins: application to the acetylcholine
receptor. J. Histochem. Cytochem. 42, 315-327 (1994).
Tzartos, S.J., Valcana, C., Kouvatsou, R., and Kokla, A. (1993) The
tyrosine phosphorylation site of the acetylcholine receptor
β-subunit is located in a highly immunogenic epitope implicated in
channel function. Antibody-probes for β subunit phosphorylation and
function. EMBO J. 12, 5141-5149.
Papadouli, I., Sakarellos, C.,and Tzartos, S.T. (1993). High
resolution epitope mapping and fine antigenic characterization of the
main immunogenic region of the acetylcholine receptor. Improving the
binding activity of synthetic analogues of the region. Eur. J. Biochem.
211, 227-234.
Eleftheriou, C.S., Trakas, N., Kokla, A. and Tzartos, S.J. (1993). A
group of three fibroblast secreted polypeptides suppressed by cellular
ageing and interferon-γ. Bioch. Bioph. Acta. 1180, 304-312.
Detsikas, E., Tsikaris, V., Sakarellos-Daitsiotis, M., Sakarellos,
C., Cung, M.T., Marraud, M., Vatzaki, E. and Tzartos, S.J. (1993).
Cyclic lactam analogues containing the main immunogenic region of
Torpedo acetylcholine receptor. Peptide Research 6, 17-23.
Tsikaris, V., Detsikas, E., Sakarellos-Daitsiotis, M., Sakarellos,
C., Vatzaki, E., Tzartos, S.J., Marraud, M. and Cung, M.T., (1993).
Conformational requirements for molecular recognition of ACh receptor
MIR analogues by monoclonal anti-MIR antibody: A 2D-NMR and molecular
dynamics approach. Biopolymers. 33, 1123-1134.
Mamalaki, A., Trakas, N., and Tzartos, S.J. (1993). Bacterial
expression of a single-chain Fv fragment which efficiently protects the
acetylcholine receptor against antigenic modulation caused by
myasthenic antibodies. Eur. J. Immunol. 23, 1839-1845.
Melms, A., Weissert, R., Klinkert, W.E.F., Schalke, B.C.G., Tzartos,
S. and Wekerle, H. (1993). Specific immune complexes augment in vitro
acetylcholine receptor-specific T-cell proliferation. Neurology 43,
583-588.
Vatzaki, E. H., Acharya, K.R., Oikonomakos, N. G. and Tzartos, S.J.
(1993). Crystallization and preliminary crystallographic study of an
Fab fragment of a pathogenic rat monoclonal antibody against the
nicotinic acetylcholine receptor. Protein Science. 2: 1770-1772.
Loutrari, H., Tzartos, S.J. and Claudio, T. (1992). Use of
Torpedo-mouse hybrid acetylcholine receptors reveals immunodominance of
the α-subunit in myasthenia gravis antisera. Eur. J. Immunol. 22,
2949-2956.
Loutrari, H., Kokla, A. and Tzartos, S.J. (1992). Passive transfer
of experimental myasthenia gravis via antigenic modulation of
acetylcholine receptor. Eur. J. Immunol. 22, 2449-2452.
Zhang, Y., and Tzartos, S.J. (1992). B-T lymphocyte interactions in
experimental autoimmune myasthenia gravis. Autoantibody mediated
up-regulation of the response of AChR-specific T-lymphocytes.
Immunology 77, 571-576.
Marx, A., Osborn, M., Tzartos, S., Geuder, K.I., Schalke, B., Nix,
W., Kirchner, T., and Muller-Hermelink, H.K. (1992). A striational
muscle antigen and myasthenia gravis-associated thymomas share an
acetylcholine receptor epitope. Develop. Immunol. 2, 77-84.
Cung, M.T., Demange, P., Marraud, M., Tsikaris, V., Sakarellos, C.,
Papadouli, I., Kokla, A., Tzartos, S.J. (1991). Two-dimentional 1H-NMR
study of antigen-antibody interactions: binding of synthetic
decapeptides to an anti-acetylcholine receptor monoclonal antibody.
Biopolymers. 31, 769-776.
Verschuuren, J.J.G.M., Graus Y.M.F., Bos N.A., Tzartos S.J., Van
Breda Vriesman P.J.C., and De Baets M.H. (1991). Paratope and framework
related idiotopes on acetylcholine receptor antibodies. J. Immunol.
146, 941-948.
Verschuuren, J.J.G.M., Graus Y.M.F., Van Breda Vriesman P.J.C.,
Tzartos S.J., and De Baets M.H. (1991). In vivo effects of neonatal
administration of anti-idiotype antibodies on experimental autoimmune
myasthenia gravis Autoimmunity. 10:173-179.
Tzartos, S. J., Loutrari, H., Tang, F., Kokla, A., Walgrave, S.,
Milius, R. P. and Conti-Tronconi, B. (1990). Main immunogenic region of
Torpedo electroplax and human muscle acetylcholine receptor.
Localization and micro-heterogeneity reveiled by the use of synthetic
peptides. J. Neurochem. 54, 51-61.
Plinkert, P.K., Gitter, A.H., Zimmermann, U., Kirchner, T., Tzartos,
S. and Zenner, H.P. (1990). Visualization and functional testing of
acetylcholine receptor-like molecules in cochlear outer hair cells.
Hearing Res. 44, 25-34.
Marx, A., O' Connor, R., Geuder, K.I., Hoppe, F., Schalke, B.,
Tzartos, S., Kalies, I., Kirchner, T., and Muller-Hermelink, H. K.
(1990). Characterization of a protein with an acetylcholine receptor
epitope from myasthenia gravis-associated thymomas. Lab. Invest.. 62,
279-286.
Tzartos, S.J., Efthimiadis, A., Morel, E. and Bach, J.F. (1990)
Neonatal myasthenia gravis: antigenic specificities of antibodies in
sera from mothers and their infants. Clin. Exp. Immunol. 80, 376-380.
Papadouli, I., Potamianos, S., Hadjidakis, I., Bairaktari, H.,
Tsikaris, V., Sakarellos, C., Cung, M.T., Marraud, M. and Tzartos, S.J.
(1990). Antigenic role of single residues within the main immunogenic
region of the nicotinic acetylcholine receptor. Biochem. J. 269,
239-245.
Tzartos, S.J and Remoundos, M.S. (1990). Fine localization of the
major α-bungarotoxin binding site to residues α189-195 of the Torpedo
acetylcholine receptor. Residues 189, 190 and 195 are indispensable for
binding. J. Biol. Chem. 265, 21462-21467. |