Tzartos, S., Cung, M., Demange, P., Loutrari, H., Mamalaki, A., Marraud, M., Papadouli, I., Sakarellos, C., Tsikaris, V. The main immunogenic region (MIR) of the acetylcholine receptor and the anti-MIR antibodies. Molecular Neurobiology, 5:1-29, 1991.

 

Mamalaki, A., Trakas, N., Tzartos, S. Bacterial expression of a single-chain Fv fragment which efficiently protects the acetylcholine receptor against antigenic modulation caused by myasthenic antibodies. European Journal of Immunology, 23:1831845, 1993.

 

Mamalaki, A. and Tzartos, S.  Nicotinic Acetylcholine Receptor: Structure, Function and Main Immunogenic Region. Advances in Neuroimmunology, 4:339-354, 1994.

 

Kioussi, C., Mamalaki, A., Mirski, R., Jessen, K., Matsas, R. Expression of endopeptidase-24.11/ (common acute lymhoblastic leukemia CD10) in the sciatic nerve in the adult rat after lesion and during regeneration. European Journal of Neuroscience, 7:951-961, 1995.

 

Mamalaki, A., Boutou, E., Patsavoudi, E., Hurel, C., Tzartos, S. and Matsas, R. BM88: cDNA cloning of a novel neuron-specific molecule from pig nervous system that accelarates the differentiation of transfected mouse neuroblastoma cells. Journal of Biological Chemistry, 270:14201-14208, 1995.

 

Tzartos, S., Cung, M., Mamalaki, Α., Marraud, M., Papanastasiou, D., Sakarellos, C., Sakarellos-Daitsiotis, M. and Tsantili, P. Antigenic Structure of the Muscle Nicotinic Acetylcholine Receptor. Annales de  Medecine Interne, 147:442-445, 1996.

 

Gaitanou, M., Mamalaki, Α., Merkouri, E. and Matsas, R. Purification and cDNA cloning of mouse BM89 antigen shows that it is identical with the synaptic vesicle protein synaptophysin. Journal of Neuroscience Research, 48:507-514, 1997.

 

Tzartos, S., Barkas, T., Cung, M., Mamalaki, A., Marraud, M., Orlewski, P., Papanastasiou, D., Sakarellos, C., Sakarellos-Daitsiotis, M., Tsantili, P. and Tsikaris, V. Anatomy of the antigenic structure of a large membrane autoantigen, the muscle-type nicotinic acetylcholine receptor. Immunological Reviews, 163: 89-120, 1998.

 

Tzartos, SJ., Tsantili, P., Papanastasiou, D., Mamalaki, A. Construction of single-chain Fv fragments of anti-MIR monoclonal antibodies. Annals of the New York Academy of Science, 841:475-7, 1998.

  

Gomez, J., Boutou, E., Hurel, C., Mamalaki, A., Kentroti, S., Vernadakis A. and Matsas, R. Overexpression of the neuron specific molecule, BM88, in mouse neuroblastoma cells: altered responsiveness to growth factors. Journal of Neuroscience Research, 51:119-128, 1998.

  

Tsantili, P., Tzartos S. and Mamalaki, A. High affinity scFv antibody fragments protecting the human nicotinic acetylcholine receptor. Journal of Neuroimmunology, 94:15-27, 1999.

  

Papanastasiou, D., Mamalaki, A., Eliopoulos, E., Poulas, K., Liolitsas, C. and Tzartos, S. Construction and characterization of a humanized single chain Fv antibody fragment against the main immunogenic region of the acetylcholine receptor. Journal of Neuroimmunology, 94:182-195, 1999.

 

Martin-Ruiz, C. M., Court, J.A., Molnar, E., Lee, M., Gotti, C., Mamalaki, A., Tsouloufis, T., Tzartos, S., Ballard, C., Perry, R.H. and Perry, E.K. Alpha 4 but not alpha 3 and 7 nicotinic acetylcholine receptor subunits are lost from the temporal cortex in Alzheimer’s disease. Journal of  Neurochemistry, 73(4):1635-40, 1999.

 

Baxevanis, C.N., Spanakos, G., Voutsas, I.F., Gritzapis, A.D., Tsitsilonis, O.E., Mamalaki, Α. and Papamichail, M. Increased generation of autologous tumor-reactive lymphocytes by anti-CD3 monoclonal antibody and prothymosin alpha. Cancer Immunology Immunotherapy, 48(2-3):71-84, 1999.

 

Kleinjung, J., Petit, M.C., Orlewski, P., Mamalaki, A., Tzartos, S., Tsikaris, V., Daitsiotis-Sakarellos, M., Sakarellos, C., Marraud, M. and Cung, M.T. The Third-Dimensional Structure of the Complex between an Fv Antibody Fragment and an Analog of the Main Immunogenic Region of the Acetylcholine Receptor: A Combined Two-Dimensional NMR, Homology and Molecular Modeling Approach. Biopolymers, 53(2):113-28, 2000.

 

Kontou, Μ., Leonidas, D.D., Vatzaki, E.H., Acharya, K. R., Mamalaki, A., Oikonomakos, N.G. and Tzartos, S.J. The crystal structure of a Fab fragment of a rat monoclonal antibody against the main immunogenic region of the human muscle acetylcholine receptor. European Journal of Biochemistry, 267(8):2389-2397, 2000.

  

Tsouloufis, T., Mamalaki, A., Remoundos, M., Tzartos, S.J. Reconstitution of conformationally dependent epitopes of the N-terminal extracellular domain of human muscle AChR α subunit expressed in E.coli: implications for myasthenia gravis therapeutic approaches. International Immunology, 12(9):1255-1265, 2000.

  

Boutou, E., Matsas, R. and Mamalaki, A. Ιsolation of a mouse brain cDNA expressed in developing neuroblasts and mature neurons.  Molecular Brain Research, 86:153-167, 2001.

  

Gaitanou, M., Buanne, P., Pappa, C., Mamalaki, A., Tirone, F. and Matsas, R. Cloning, expression and localization of human BM88 shows that it maps to chromosome 11p15.5, a region implicated in Beckwith-Wiedemann syndrome and tumorigenesis, Biochemistry Journal 355:715-724, 2001.

  

Perez, S., Sotiropoulou, P., Sotiriadou, N., Mamalaki, A., Gritzapis, A., Echner, H., Voelter, W., Pawelec, G., Papamichai,l, M., Baxevanis, CN. HER-2/neu-derived peptide 884-899 is expressed by human breast, colorectal and pancreatic adenocarcinomas and is recognized by in-vitro-induced specific CD4(+) T cell clones. Cancer Immunol Immunotherapy 50(11):615-24, 2002.

   

Sotιriadou, N., Perez, S., Gritzapis, A., Sotiropoulou PA, Echner, H., Heinzel, S., Mamalaki, A., Pawelec, W., Voelter, W., Baxevanis, CN., and Papamichail, M. Peptide HER-2/neu (776-788) represents a, naturally processed broad MCH class II restricted T cell epitope. British Journal of Cancer 85(10), 1527-1534, 2001

   

Psaridi, L., Mamalaki, A., Remoundos M., and Tzartos, S. Expression of soluble ligand- and antibody-binding extracellular domain of human muscle acetylcholine receptor alpha subunit in yeast Pichia pastoris. Role of glycosylation in alpha -bungarotoxin binding. J Biol Chem. 277, 30, 26980-26986, 2002.

  

Gritzapis A., Mamalaki A., Kretsovali Α., Papamatheajis J., Belimezi Μ., Perez S., Baxevanis C. and Papamichail M. Redirecting mouse T hybridoma against human breast and ovarian carcinomas: in vivo activity against HER-2/neu expressing cancer cells. Br J Cancer. 2003 Apr 22; 88(8):1292-300.

  

Mamalaki A., Gritzapis A., Kretsovali Α., Belimezi Μ., Papamatheajis J., Perez S., Papamichail M and Baxevanis C. In vitro and in vivo antitumor activity of a mouse CTL hybridoma expressing chimeric receptors bearing the single chain Fv from HER-2/neu- specific antibody and the γ-chain from Fc(ε) RI. Cancer Immunol Immunother. 52(8): 513-22, 2003.

   

Psaridi-Linardaki, L., Mamalaki, A., and Tzartos, S. Future therapeutic strategies in autoimmune Myasthenia Gravis. Annals of the New York Academy of Science, 998: 539-548, 2003.

   

V. Avramopoulou, A. Mamalaki and S. Tzartos. Soluble oligomeric and ligand-binding extracellular domain of human neuronal alpha7 acetylcholine receptor expressed in the yeast Pichia pastoris: Replacement of the hydrophobic Cys-loop by the hydrophilic loop of ACh-binding protein enhances protein solubility. J. Biol.Chem., 279(37):38287-93.  2004.

  

Loukia Psaridi-Linardaki, Nikos Trakas, Avgi Mamalaki and Socrates J. Tzartos Specific immunoadsorption of the autoantibodies from myasthenic patients using the extracellular domain of the human muscle acetylcholine receptor α-subunit. Development of an antigen-specific therapeutic strategy.  Journal of Neuroimmunology, 159:183-191, 2005.
 

Fostieri E, Tzartos SJ, Berrih-Aknin S, Beeson D, Mamalaki A. Isolation of potent human Fab fragments against a novel highly immunogenic region on human muscle acetylcholine receptor which protect the receptor from myasthenic autoantibodies. Eur J Immunol. 35: 632–643, 2005.