Document for booking the microscope
                          

Responsible researchers:

Haralabia Boleti, PhD
Parasitology unit
Email: hboleti@pasteur.gr
Tel. +33 210 6478879

The Light Microscopy Unit of the Hellenic Pasteur Institute is one of the most modern Imaging units in Greece and is supported by investigators with strong experience in Imaging Techniques. It is equipped with modern microscopes amongst which 

1)a four channels (3 fluorescence +1 transmission light) Confocal Microscope Leica TCS-SP (http://www.confocal-microscopy.com/) equipped with Argon Ion and Helium-neon Lasers. The confocal system operates with the Leica Confocal Software LCS, which allows for 2D measurements as well as for 3D image reconstructions.

2)a Live Cell Imaging System, composed of a wide field inverted Olympus IX-81 microscope equipped with hardware for FRET, a Hamamatsu Orca cryocamera and the Cell R imaging software, which allows  time lapse, spectral unmixing, FRET and 3D-dencovolution analysis.

The Light microscopy unit is also equipped with two Image analysis workstations with RAM memory > 3G and softwares (i.e. Imaris, Image Pro) for analysis of the digital data obtained by the confocal microscope and the Olympus Cell R system.

A small tissue culture facility consisting of a BSL2 laminar flow hood and a CO₂ incubator is also set up within the imaging unit c.

Both the confocal microscope and the wide field live imaging system are widely used by scientists of the HPI to perform studies in the fields of Microbiology, Cell Biology and Neurobiology that allow double and triple co-localization of molecules in both cellular and sub-cellular levels and imaging of live cells in 4D and 5D.

The Light microscopy unit of HPI collaborates closely with the Platform for Dynamic Imaging of the Pasteur Institute-Paris (Plate-forme d’imagerie Dynamique(http://www.pfid.org/html/pres/?fr) where scientists of the HPI are trained and technical expertise in a number of imaging techniques has been obtained.

The Leica confocal microscope and the Olympus X81 inverted microscope of the HPI Light Microscopy unit are available for use by external users from other Greek Research centers and Universities under a symbolic charge / hour of use.
 
Finally the HPI Light Microscopy unit has organized two workshops in modern Light Microscopy techniques supported by the GSRT program “Human Networds for Training in Research and Technology”.