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HPI seminars 2008 - 2009    06/02/2009



Hellenic Republic
Ministry of Development
General Secretariat
for Research and Technology
 
127, Vas. Sofias Avenue 11521,
Athens, Greece 
Τel: (+30) 210 6478 800
 Fax: (+30) 210 6423 498
 
 
Equipment
 

 

:: Unit :: Workshops :: Regulations :: Gallery ::

 

:: Document for booking the microscope ::
                            


:: Workstation 1 :: Workstation 2 :: Workstation 3 :: Workstation 4 :: Plan of the unit ::

 

Workstation 1
Leica TCS-SP confocal Microscope
(http://www.confocal-microscopy.com/)

This is an older version of the Leica laser scanning confocals. However the measurement software is identical to the one on the Leica SP2 AOBS system.

Microscope
DMR upright microscope
FITC and Rhodamine filter sets (for conventional epi-illmination).

Objectives
  10x 0.3   PH1   HC PL Fluotar     dry
  20x 0.7   PH2   HC PlanApo        dry
  40x 0.75-1.25   HCX PLApoCS   oil
  63x 0.6-1.32     HCX PLApo        oil

Lasers 
  Argon:     458, 488, 514 nm
  He/Neon: 543, 633 nm

Detectors
  3  fluorscence  PMT-s (spectral detection option)
  1  transmission PMT

Image acquisition and analysis software
            Leica LCS

 

 
 

 

Workstation 2 –Image analysis
(Leica LCS, Image Pro, Photoshop)

 

 

Workstation 3

Time lapse Olympus X81 Cell-R
The Olypmus Cell-R is a fully motorized epi-flurescence microscope equipped with a fast excitation filter wheel allowing for fast multicolor 3D imaging.
The 3D datasets can further be deconvolved with Deconvolution software (Cell R or Imaris). An on line Imaris software is installed in Workstation 3

Stage
  Olympus IX8S1F-ZDC (inverted), equipped for DIC and phase contrast imaging.

Filters
  3 filter sets (open page with filter combinations)
  1) DAPI, FITC, TXRed (and their combination for fast imaging)
  2) DAPI, FITC, C3, C5
  3) CFP, YFP

Objectives
  10x 0.3   NA Ph1 UPlanFLN  dry
  20x 045  NA LUCPLanFLN   dry
  40x 0.6   NA LUCPLanFLN   dry
  60x 1.42 ΝΑ PlanApo           oil
  60x 1,2   NA UPLSApo         water
  100x 1.4 ΝΑ UPLSApo         oil

Temperature and CO2 chamber

Camera
  CCD ORCA/AG  Hamamatsu

 

 

 

 

Workstation 4 - Image Analysis (Olympus Cell R, Imaris)

 

 

 
 



Facility Plan


 
 
 
 
 
http://www.b2b12.com/