Parasite Immunology

Dr Evdokia Karagouni |Department of Microbiology

Research Director

Contact:

+30 210-6478826 | .img@.img |  CV

ABOUT

The Group of Parasite Immunology belongs to the Laboratory of Cellular Immunology of the Department of Microbiology of HPI, and is active in the field of parasitic infections. In particular, the research interest of the group focuses on:

  • the analysis of immunopathogenic mechanisms that dictate the development of the disease in order to determine biomolecules for the development of immunotherapies and
  • the detection of macromolecules or antigenic determinants of the pathogen with potential use in the development of vaccines or diagnostics.

So far, we have developed know-how in the manufacture of peptide-based nanovaccines, which have the ability to induce protection in the experimental model of visceral leishmaniasis. In parallel, by analyzing the underlying immunological mechanisms governing protection versus susceptibility to infection, we have identified a set of biomarkers, which can be used as molecular targets for immunotherapy for the enhancement of candidate vaccines effectiveness.

UNITS

A. Immunology of Leishmaniasis and Vaccine Development

Leishmaniasis is a vector-borne diseases complex caused by protozoan parasites of the genus Leishmania. Parasites are transmitted by species of Phlebotomine sand flies as extracellular, flagellated promastigotes and replicate in the form of intracellular, non-flagellate amastigotes in the mononuclear phagocytes of mammalian hosts (humans, dogs). Leishmaniasis range from self-healing, asymptomatic infection to localized skin lesions, and can be developed into a life-threatening progressive visceral form of disease. Epidemiological data show that visceral leishmaniasis (VL) is the second most common parasitic cause of death among tropical infections affecting mainly very poor people in tropical and subtropical regions of the world. Despite the fact that several clinical data indicate that protection from disease can be achieved in most of the people through vaccination and a significant number of vaccine candidates have been successfully evaluated in animal models of the disease, there is still no efficacious vaccine in use for humans. During the last few years, system vaccinology approaches have added in the understanding of Leishmania infection pathogenesis and the generation of host-protective immunity, providing new roadmaps in vaccine research.

A1. Construction of nanovaccines for efficient dendritic cells targeting

Vaccine efficacy to induce the desired immune responses is largely dependent not only on the included antigen and adjuvant, but also on vaccine’s morphological properties and particle size. During the last few years the development of nano-sized vaccines is considered one of the most advantageous approaches for vaccine development due to their unique properties, including enhanced uptake by APCs, protection of the incorporated antigen from metabolism and elimination, low toxicity, as well as immunostimulatory properties based on their size. Among them, poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) are often used as first choice for polymeric nanovaccine delivery systems mainly due to their excellent safety profile and established use in commercial products for controlled drug delivery. Based on these, our group is interested in the development of nanovaccine candidates against leishmaniasis. For this purpose, we collaborate with chemical engineering labs for synthesis/construction of various multi-functionalized biodegradable NPs that are selectively modified with the encapsulation of

adjuvants, e.g. MPLA, CpG ODNs for the enhancement of the induced immune responses or are surface-modified for the efficient targeting of DCs.  As such, encouraging first data from our group showed that mice vaccination with multi-functionalized PLGA NPs fortified with soluble L. infantum antigens confers significant levels of protection against experimental VL opening the way for the construction of more sophisticated and rationalized anti-leishmanial nanovaccines.

Related Publications

  • Margaroni, M., M. Agallou, E. Athanasiou, O. Kammona, C. Kiparissides, C. Gaitanaki, E. Karagouni (2017). Vaccination with PLGA nanoparticles loaded with soluble Leishmania antigens and modified with a TNFα mimicking peptide or MPLA confers protection against experimental visceral leishmaniasis. International Journal of Nanomedicine, In press.
  • Margaroni, M., M. Agallou, K. Kontonikola, K. Karidi, O. Kammona, C. Kiparissides, C. Gaitanaki and E. Karagouni (2016). PLGA nanoparticles modified with a TNFα mimicking peptide, soluble Leishmania antigens and MPLA induce T cell priming in vitro via dendritic cell functional differentiation. European journal of Pharmaceutics and Biopharmaceutics 105, 18-31.

A2. Identification of novel antigens for the development of peptide-based vaccines

The minimalistic composition of defined subunit vaccines consisted of recombinant proteins or specific peptides benefits over the classic vaccines based on attenuated or inactivated pathogens, due to the fact that are produced easily, they are non-infectious and they can include several epitopes targeting different stages in the life cycle or subtypes of a pathogen. Previous studies from our group show that experimental cellular vaccination with dendritic cells (DCs) sensitized with parasite antigenic molecules, such as Histone H1 or selected peptides of KMP-11 confers significant levels of protection in BALB/c mice infected with visceral Leishmania strains, mediated by CD4+ TH1-type immune responses. The existence of asymptomatic infected dogs (AD) in endemic areas of VL, among them Greece, further supports the idea of developing an effective vaccine against VL, since IgG-dominated antibody responses in AD are associated with protective cell-mediated immunity. Moreover, knowledge of immunodominant serospecificities in these dogs leads to the identification of Leishmania antigens that could be considered as vaccine candidates due to their protective potential against re-infection. By combining immunoproteomics and bioinformatics analysis, we have identified 7 proteins that were exclusively recognized by AD sera and were also found to be highly immunogenic in terms of T cells epitope content against the most frequent HLA alleles in the human population. Moreover, by using in silico approach only, we have identified several MHC class I and II restricted epitopes from different Leishmania proteins and evaluated their protective potential in the experimental VL, while in silico analysis of whole Leishmania genome – in order to identify new antigenic proteins for the rational design of a more effective in terms of protection peptide-based vaccine or to develop more sensitive diagnostic tests – is underway.

Related Publications

  • Athanasiou, E., M. Agallou, S. Tastsoglou, O. Kammona, A. Hatzigeorgiou, C. Kiparissides, E. Karagouni (2017). Chimeric peptides containing HLA class I-restricted epitopes from Leishmania infantum proteins encapsulated in PLGA nanoparticles with MPLA induce functional differentiation of dendritic cells and promote peptide-specific IFNg-producing CD8+ T cells. Frontiers in Immunology, 8:684, doi.org/10.3389/fimmu.2017.00684.
  • Agallou, M., M. Margaroni, E. Athanasiou, D.K. Toubanaki, K. Kontonikola, K. Karidi, O. Kammona, C. Kiparissides, E. Karagouni (2017). Identification of BALB/c Immune Markers Correlated with a Partial Protection to Leishmania infantum after Vaccination with a Rationally Designed Multi-epitope Cysteine Protease A Peptide-Based Nanovaccine. PLoS Neglected Tropical Diseases, 11 (1), e0005311.
  • Agallou, M., E. Athanasiou, M. Samiotaki, G. Panayotou and E. Karagouni (2016). Identification of Immunoreactive Leishmania infantum Protein Antigens to Asymptomatic Dog Sera through Combined Immunoproteomics and Bioinformatics Analysis. PLoS One 11(2), e0149894.
  • Agallou, M., E. Athanasiou, O. Koutsoni, E. Dotsika and E. Karagouni (2014). Experimental Validation of Multi-Epitope Peptides Including Promising MHC Class I- and II-Restricted Epitopes of Four Known Leishmania infantum Frontiers in Immunology 5:268, doi: 10.3389/fimmu.2014.00268.
  • Agallou, M., D. Smirlis, K. Soteriadou and E. Karagouni (2012). Vaccination with Leishmania histone H1-pulsed dendritic cells confers protection in murine visceral leishmaniasis. Vaccine 30(34), 5086-5093.
  • Agallou, M., M. Margaroni and E. Karagouni (2011). Cellular vaccination with bone marrow-derived dendritic cells pulsed with a peptide of Leishmania infantum KMP-11 and CpG oligonucleotide induces protection in a murine model of visceral leishmaniasis. Vaccine 29(31), 5053-506.

A3. Host-pathogen Interaction: genome analysis for the detection of specific biomarkers related to disease sensitivity or vaccine protective immunity

A necessary precursor for design new as well as to improve the already existing vaccines is to better understand the fundamental mechanisms underlying immunosenescence. The growing field of systems vaccinology combines modern analytic tools of systems biology with data on immunological responses patterns to pathogens and vaccines, in order to uncover molecular signatures of vaccine efficacy and to guide the design and evaluation of new vaccines. Hence, we are undertaking a series of molecular approaches (RNA-seq, microarrays) in combination with functional analyses to uncover the transcriptional activity of the immune response against Leishmania by using in vitro and in vivo infection systems. The ultimate goals are to extent the understanding and provide new insights into the immunobiology of leishmaniasis. First data obtained by microarrays analysis on transcriptome from DCs ex vivo exposed to different nanovaccine candidates revealed several markers related to vaccine efficacy. Moreover, at the current period we are trying to elucidate the immune response profile associated with the pathology in the spleen in the experimental model of VL using vaccinated and challenged with L. infantum mice as a comparative model system. Furthermore, we are conducting a dual high-throughput analysis on host-pathogen interaction using DCs exposed to L. infantum. By these means we are planning to define inherent and infection-mediated perturbations in the genetic regulatory networks of the host and this will enable us to define biomarkers for the prediction about how the host will respond to candidate vaccines and/or molecules for the design of vaccines that induce optimal immune responses.

Related Publications

  • Athanasiou, E., M. Agallou, S. Tastsoglou, O. Kammona, A. Hatzigeorgiou, C. Kiparissides, E. Karagouni (2017). Chimeric peptides containing HLA class I-restricted epitopes from Leishmania infantum proteins encapsulated in PLGA nanoparticles with MPLA induce functional differentiation of dendritic cells and promote peptide-specific IFNg-producing CD8+ T cells. Frontiers in Immunology, 8:684, doi.org/10.3389/fimmu.2017.00684.

B. Development of Low-Cost Diagnostics for Infectious Diseases

The use of molecular diagnostics, especially of biosensors, is of great importance, since it has been shown that an early diagnosis can change dramatically the development of a disease or prevent epidemics. Nanomaterials are bringing important advantages to the design of novel biosensing systems or improvements of the existing methodologies. Paper-based nano-biosensors are an excellent example of low-cost sensors developed as the result of the synergy between nanotechnology and biosensing technology. Our research group is actively interested in development and validation of novel nanoparticle-based lateral flow biosensors (LFBs) for pathogen detection. Nano-biosensors for fish nervous necrosis virus and Leishmania detection have been successfully developed and our on-going efforts are focused on commercial exploitation of the research results. Extensive research for LFBs optimization has, already, led in signal enhancement methods development, with application in several biosensor formats. Moreover, development of novel molecular methodologies for virus detection and characterization with applications in basic research and the aquaculture industry with great economic implications for the environmental and food sectors are among our main interests. In parallel, our group studies the functionalization of gold and metallic nanoparticle formulations with antibodies and oligonucleotides. Nanoparticle-enzymes (nanozymes) are quite attractive for immunoassays development with low-cost and high stability. Currently, magnetic graphene-based nanocomposites have been characterized as nanozymes for use as labels in immunoassays and DNA detection protocols, with very promising results.

Related Publications

  • Toubanaki, D.K. and E. Karagouni (2017). Genotype-specific real-time PCR combined with high-resolution melting analysis for rapid identification of red-spotted grouper nervous necrosis virus. Archives of Virology, doi: 10.1007/s00705-017-3375-4.
  • Toubanaki, D.K., E. Athanasiou and E. Karagouni (2016). Gold nanoparticle-based lateral flow biosensor for rapid visual detection of Leishmania amplification products. Journal of Microbiological Methods 127, 51–58.
  • Toubanaki, D.K., M. Margaroni and E. Karagouni (2016). Dual enhancement with a nanoparticle-based lateral flow biosensor for the determination of DNA. Analytical Letters 49(7), 1040-1055.
  • Toubanaki, D.K. and E. Karagouni (2016). Oligonucleotide-conjugated gold nanoparticles for application on lateral flow biosensors: Evaluation and optimization of low pH and salt-aging conjugation methods. Analytical Letters 49 (17), 2833-2850.
  • Toubanaki, D.K., M. Margaroni and E. Karagouni (2015). Nanoparticle-based lateral flow biosensor for visual detection of fish nervous necrosis virus amplification products. Molecular & Cellular Probes 29,158-166.
  • Toubanaki, D.K., M. Margaroni and E. Karagouni (2015). Development of a novel allele-specific PCR method for rapid assessment of Nervous Necrosis Virus genotypes. Current Microbiology 71,529-539.

C. Fish immunology / Evaluation of Alternative Medications

Understanding the pathology of infectious diseases affecting the aquaculture is of high priority, since aquaculture is an important sector of the Greek and European economy. Plant or bacterial organism extracts are used as dietary supplements to promote fish growth, or alternatively act as antimicrobial agents, since chemically-originated parasiticides are rare or virtually unavailable, while antibiotics have a negative impact on the quality of fish as food, and on the aquatic environment. As part of research consortia with academic institutions and fish farming industries, we are interested to: (i) characterize the immunological responses elicited in natural or experimentally induced parasitic diseases of fish species with commercial interest in Mediterranean aquaculture; (ii) determine specific biomarkers with prognostic value and (iii) evaluate alternative treatments against the most common fish parasitoses. According to our latest results, infection of sea bass with b-nodavirus responsible for viral encephalopathy and retinopathy, leads to the overexpression of several genes associated with fish innate and adaptive immunity, while fish fed with diets supplemented with oregano essential oil seems to be more resistant to the infection. In addition, molecular and cellular methods have been developed for the diagnosis and the massive propagation of the virus.

Related Publications                                                        

  • Giannenas, I., I. Karamaligas, M. Margaroni, I. Pappas, E. Mayer, P. Encarnação and E. Karagouni (2015). Effect of dietary incorporation of a multi-strain probiotic on growth performance and health status in rainbow trout (Oncorhynchus mykiss). Fish Physiology & Biochemistry 41(1), 119-28.
  • Rigos, G., E. Karagouni, I. Kyriazis, E. Athanasiou, K. Grigorakis, E. Kotou and P. Katharios (2013). In vitro and in vivo evaluation of quinine in gilthead sea bream, Sparus aurata naturally infected with the ciliate Cryptocaryon irritans. Aquaculture 416-417, 185-91.

Giannenas, I., E. Triantafillou, S. Stavrakakis, M. Margaroni, S. Mavridis, T. Steiner and E. Karagouni (2012). Assessment of dietary supplementation with carvacrol or thymol containing feed additives on performance, intestinal microbiota and antioxidant status of rainbow trout (Oncorhynchus mykiss). Aquaculture 350-353, 26-32.

 

 

 

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SERVICES - FUNDING

2017-2019 Stavros Niarchos Foundation

Development of innovating Biological Products and Health Services targeting Infectious and Neurodegenerative diseases (PI: Dr. R. Matsa & Dr. P. Miriagou)

 

2016 HPI Postdoctoral FellowshipS

Development of chimeric peptide-based vaccines

Scientific Director: Dr. E. Karagouni

Post-doctoral Fellow: Dr. E. Athanasiou

 

2015-2017 GSRT / SIEMENS

Development of Next Generation Sequencing Technology aiming to improved diagnosis, Laboratory surveillance and control of Infecrious Diseases (PI: Prof. Μ. Μanousakis)

 

2014 EMBO/RIIP

Bioinformatics and Genome Analysis Practical Course

Organisers: HPI (Dr. E. Karagouni & Prof. M. Manoussakis) and PI (Dr. F. Tekaia & Dr. E. Melanitou)

 

2014-2016 IKY Fellowships of Excellence for Postgraduate Studies in Greece-Siemens Program Biomarkers’ identification for the preclinical evaluation of prophylactic or/and therapeutic vaccines against visceral leishmaniasis

Scientific Director: Dr. E. Karagouni

Post-doctoral Fellow: Dr. M. Agallou

 

2012-2015 GSRT, ESF/NSFR 2007-2013 – Action “PROPOSALS FOR RESEARCH CENTERS’ DEVELOPMENT – KRIPIS”

Infectious and neurodegenerative diseases in the 21st century: study of basic mechanisms for thedevelopment of the translational research and cutting edge technologies aiming to effective diagnosis prevention and therapy (MIS 450498), (PI: Dr. E. Karagouni)

 

2012-2015 GSRT, ESF/NSFR 2007-2013 – Action “THALIS”

Analysis of mechanisms of immunopathogenesis in natural and experimental infections of fish with Nodavirus: importance of the infection for public health (MIS 375267).

Coordinator: University of Thessaly (PI: Prof. F. Athanassopoulou)

Partners: University of Thessaloniki (Prof. M. Papanastasopoulou), HPI/HCMR/University of Aegean (Dr. E. Karagouni)

 

2012-2015 GSRT, ESF/NSFR 2007-2013 – Action “Supporting Postdoctoral Researchers ”

Development of lateral flow biosensors for virus, viral antibodies and nucleic acids detection, as a complete analysis system for environmental-threatening viruses: application on fish nervous necrosisvirus (Nodavirus) as a model system (LS9-448).

Scientific Director: Dr. E. Karagouni

Post-doctoral Fellow: Dr. D.Toubanaki

 

2011-2014 GSRT, ESF/NSFR 2007-2013 – Action “COOPERATION”

Synthesis of polymeric nanocarriers for targeted antigen delivery to dendritic cells (09SYN-14-643)

Coordinator: HPI (PI: Dr. E. Karagouni),

Partners: CERTH (Prof. C. Kiparissides), PROVET SA (Dr. N. Korbis).

 

 

2011-2013 GSRT, ESF/NSFR 2007-2013 – Action “COOPERATION”

Alternative preventive and therapeutic measures in Greek mariculture (09ΣΥΝ-24-323).

Coordinator: HCMR (PI: Dr. G. Rigos)

Partners: HPI (Dr. E. Karagouni), SELODA SA (I. Zarkadas), BIOMAR SA (I. Karakostas).

TRAINING

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REGULATIONS

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COURCES

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PUBLICATIONS

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Margaroni, M., M. Agallou, E. Athanasiou, O. Kammona, C. Kiparissides, C. Gaitanaki, E. Karagouni (2017). Vaccination with PLGA nanoparticles loaded with soluble Leishmaniaantigens and modified with a TNFα mimicking peptide or MPLA confers protection against experimental visceral leishmaniasis. International Journal of Nanomedicine, In press.

Athanasiou, E., M. Agallou, S. Tastsoglou, O. Kammona, A. Hatzigeorgiou, C. Kiparissides, E. Karagouni (2017). Chimeric peptides containing HLA class I-restricted epitopes from Leishmaniainfantum proteins encapsulated in PLGA nanoparticles with MPLA induce functional differentiation of dendritic cells and promote peptide-specific IFN-producing CD8+ T cells. Frontiers in Immunology 8:684, doi.org/10.3389/fimmu.2017.00684.

Agallou, M., M. Margaroni, E. Athanasiou, D.K. Toubanaki, K. Kontonikola, K. Karidi, O. Kammona, C. Kiparissides, E. Karagouni (2017). Identification of BALB/c Immune Markers Correlated with a Partial Protection to Leishmaniainfantum after Vaccination with a Rationally Designed Multi-epitope Cysteine Protease A Peptide-Based Nanovaccine. PLoS Neglected Tropical Diseases, 11 (1), e0005311.

Toubanaki, D.K. and E. Karagouni (2017).Genotype-specific real-time PCR combined with high-resolution melting analysis for rapid identification of red-spotted grouper nervous necrosis virus. Archives of Virology, doi:10.1007/s00705-017-3375-3384.

2017
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Margaroni, M., M. Agallou, K. Kontonikola, K. Karidi, O. Kammona, C. Kiparissides, C. Gaitanaki and E. Karagouni (2016). PLGA nanoparticles modified with a TNFα mimicking peptide, soluble Leishmania antigens and MPLA induce T cell priming in vitro via dendritic cell functional differentiation. European journal of Pharmaceutics and Biopharmaceutics 105, 18-31.

Sklavounos, A.A., E.K. Pefkianakis, D.K. Toubanaki, G.C. Vougioukalakis, and A.C. Calokerinos (2016). A Squaraine Derivative for Cost-Effective, Quick, and Highly Sensitive Determination of Mercury and Thiols and pH Sensing. Chem Plus Chem 81, 913-916.

Toubanaki, D.K., E. Athanasiou and E. Karagouni (2016).Gold nanoparticle-based lateral flow biosensor for rapid visual detection of Leishmania-specific DNA amplification products.Journal of Microbiological Methods 127, 51-58. Toubanaki D.K. and E. Karagouni (2016). Oligonucleotide-Conjugated Gold Nanoparticles for Application on Lateral Flow Biosensors: Evaluation and Optimization of Low pH and Salt-aging Conjugation Methods. Analytical Letters 49 (17), 2833-2850.

Toubanaki, D.K., M. Margaroni and E. Karagouni (2016). Dual Enhancement with a Nanoparticle-Based Lateral Flow Biosensor for the Determination of DNA. Analytical Letters 49 (7), 1040-1055.

Agallou, M., E. Athanasiou, M. Samiotaki, G. Panayotou and E. Karagouni (2016). Identification of Immunoreactive Leishmania infantum Protein Antigens to Asymptomatic Dog Sera through Combined Immunoproteomics and Bioinformatics Analysis. PLoS One 11(2), e0149894.

2016
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Toubanaki, D.K., M. Margaroni and E. Karagouni (2015). Nanoparticle-Based Lateral Flow Biosensor for Visual Detection of Fish Nervous Necrosis Virus Amplification Products. Molecular & Cellular Probes 29(3), 158-166.

Pefkianakis, E.K., T.A. Theodossiou, D.K. Toubanaki, E.Karagouni, P. Falaras, K. Papadopoulos, and G.C. Vougioukalakis (2015). A family of potent Ru (II) Photosensitizers with enhanced DNA intercalation: bimodal photokillers. Photochemistry and Photobiology 91(5), 1191-1202.

Toubanaki, D.K., M. Margaroni and E. Karagouni (2015). Development of a novel allele-specific PCR method for rapid assessment of Nervous Necrosis Virus genotypes. Current Microbiology 71, 529-539.

Giannenas, I., I. Karamaligas, M. Margaroni, I. Pappas, E. Mayer, P. Encarnação and E. Karagouni (2015). Effect of dietary incorporation of a multi-strain probiotic on growth performance and health status in rainbow trout (Oncorhynchus mykiss). Fish Physiol Biochem. 41:119–128.

2015
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Agallou, M., E. Athanasiou, O. Koutsoni, E. Dotsika and E. Karagouni (2014). Experimental Validation of Multi-Epitope Peptides Including Promising MHC Class I- and II-Restricted Epitopes of Four Known Leishmania infantum Proteins. Frontiers in Immunology 10(5), 268. doi: 10.3389/fimmu.2014.00268.

Agallou, M., E. Dotsika and E. Karagouni (2014). Low CD40 Expression Levels in Leishmania infantum-Infected Bone Marrow Dendritic Cells Evoke Regulatory Responses by Down-Regulating Interleukin-12 Production: Role of ERK1/2. European Journal of Inflammation 12(2), 315-328.

Agallou, M., E. Dotsika, S. Frydas and E. Karagouni (2014). Toll-like receptor 4 promotes control of Leishmania infantum infection through inducement of leishmanicidal activity in host macrophages: role of mitogen activated kinases. Journal of Biological Regulators and Homeostatic Agents 28(1), 41-52.

2014
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Karagouni, E., O. Kammona, M. Margaroni, K. Kotti, V. Karageorgiou, C. Gaitanaki and C. Kiparissides (2013). Uptake of BSA-FITC loaded PLGA Νanoparticles by Bone Marrow-Derived Dendritic Cells induce maturation but not IL-12 or IL-10 production. Nanoscience & Nanotechnology Letters 5(4), 498-504.

2013
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Giannenas, I., E. Triantafillou, S. Stavrakakis, M. Margaroni, S. Mavridis, T. Steiner and E. Karagouni (2012). Assessment of dietary supplementation with carvacrol or thymol containing feed additives on performance, intestinal microbiota and antioxidant status of rainbow trout (Oncorhynchus mykiss). Aquaculture 350-353, 26-32.

Agallou, M., D. Smirlis, K. Soteriadou and E. Karagouni (2012). Vaccination with Leishmania histone H1-pulsed dendritic cells confers protection in murine visceral leishmaniasis. Vaccine 30(34), 5086-5093.

2012
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Agallou, M., M. Margaroni and E. Karagouni (2011). Cellular vaccination with bone marrow-derived dendritic cells pulsed with a peptide of Leishmania infantum KMP-11 and CpG oligonucleotide induces protection in a murine model of visceral leishmaniasis. Vaccine 29(31), 5053-5064.

2011
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Symeonidou I., A. Kourelis, I. Frydas, E. Karagouni, A. Anogeianaki, M. Hatzistilianou and S. Frydas (2010). Modulation of NF-kappaΒ signalling pathways by parasites. Journal of Biological Regulators & Homeostatic Agents 24(4), 471-479.

Symeonidou I., M. Hatzistilianou, E. Papadopoulos, C.I. Dovas, E. Karagouni, S. Pappa, C.N. Pantzartzi, A. Kourelis and S. Frydas (2010). Susceptibility and resistance to canine leishmaniosis is associated to polymorphisms of the canine TNF-α gene. European Journal of Inflammation 9(1), 23-29.

Symeonidou I., S. Pappa, A. Kourelis, A. Anogeianaki, I. Frydas, E. Karagouni, M. Hatzistilianou (2010). Microarray analysis of NF-kappaB signaling pathways in PBMC of mice infected by Trichinella spiralis. International Journal of Immunopathology & Pharmacology 23(3), 821-831.

Tsatchev, I., I.D. Kyriazis, S. Boutsini, E.E. Karagouni and E.Ν. Dotsika (2010). First report of canine visceral leishmaniasis in Bulgaria. Turkish Journal of Veterinary Animal Science 34(5), 465-469.

2010
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Daoudaki, M., A. Diakou, S. Frydas, I. Fouzas, E. Karagouni, N. Vavatsi, and S. Haralabidis (2009). Vaccination with Trichinella spiralis antigens increase CD8+ peripheral T cells and enhancethe TH2 immune response in Leishmaniainfantum challenged mice. International Journal of Immunopathology & Pharmacology 22(1), 169-174.

Golomazou, E., F. Athanassopoulou, E. Karagouni, L. Kokkokiris (2009). The effect of seasonality on the health and growth of a newly recorded Myxobolus species infecting cultured sharpsnout seabream (Diplodus puntazzo C.). Turkish Journal of Veterinary and Animal Sciences 33(1), 1-5.

Golomazou, E., F. Athanassopoulou, E. Karagouni, P. Tsagozis, H. Tsantilas and S. Vagianou (2006). Experimental transmission of Enteromyxum leei Diamant, Lom &Dykova, 1994 in sharpsnout seabream, Diplodus puntazzo C. and the effect on some innate immune parameters. Aquaculture 260, 44-53.

Karagouni, E., F. Athanassopoulou, S. Vagianou, H. Tsantilas, and D. Karamanis (2006). Efficacy and toxicity of orally administrated anti-coccidial drug treatment on Enteromyxum leei infections in sharpsnout seabream (Diplodus puntazzo C.). Israel Journal of Aquaculture-Bamidgeh 58(3), 157-169.

Di Giannantonio, M., S. Frydas, D. Kempuraj, E. Karagouni, M. Hatzistilianou, C.M.V. Conti, W. Boucher, N. Papadopoulou, J. Donelan, J. Cao, B. Madhappan, P. Boscolo, C. Petrarca, L. Castellani, R. Quartesan, R. Doyle and F.M. Ferro (2005). Cytokines in stress. International Journal of Immunopathology & Pharmacology 18(1), 1-5.

Karagouni, E., F. Athanassopoulou, A. Lytra, C. Comis and E. Dotsika (2005). Antiparasitic and immunomodulatory effect of innovative treatments against Myxobolus sp. infection in Diplodus puntazzo. Veterinary Parasitology 134(3-4), 215-228.

Tsagozis, P., E. Karagouni and E. Dotsika (2005). Function of CD8+ T lymphocytes in a self-curing model of visceral leishmaniasis. Parasitology International 54(2), 139-146.

Karagouni, E., F. Athanassopoulou, P. Tsagozis, E. Ralli, T. Moustakareas, K. Lytra and E. Dotsika (2005). The impact of a successful anti-myxosporean treatment on the phagocyte functions of juvenile and adult Sparus aurata L. International Journal of Immunopathology & Pharmacology 18(1), 121-132.

Frydas, S., N. Papaioannou, M. Papazahariadou, M. Hatzistilianou, E. Karagouni, M. Trakatelli, G. Brellou, C. Petrarca, M.L. Castellani, P. Conti, G. Riccioni, A. Patruno and A. Grilli (2005). Inhibition of MCP-1 and MIP-2 chemokines in murine trichinellosis: effect of the anti-inflammatory compound L-mimosine. International Journal of Immunopathology & Pharmacology 18(1), 85-93.

Frydas, S., E. Karagouni, E. Papadopoulos, M. Hatzistiliannou, S. Pappas and N. Papaioannou (2004). Chemokines and their role in parasitic diseases. European Journal of Inflammation 2(2), 53-56.

Frydas, S., E. Karagouni, T. Iezzi, D. Kempuraj, C.M.V. Conti, W. Boucher, N.G. Papadopoulou, J. Donelan, B. Madhappan, R. Letourneau, G. Carratelli, A. Grilli, F. Conti, G. Riccioni, D. Tripodi, F. Meneghini, I. Stellin and G. Sabatino (2004). Human genome project and parasitic infections. European Journal of Inflammation 2(1), 1-3.

Frydas, S., E. Karagouni, M. Hatzistilianou, D. Kempuraj, S. Comani, C. Petrarca, T. Iezzi, N. Verna, P. Conti and M.L. Castellani (2004). Cytokines and allergic disorders: Revisited study. International Journal of Immunopathology & Pharmacology 17(3), 233-235.

Tsagozis, P., V. Tseveleki, L. Probert, E. Dotsika and E. Karagouni (2004). Vaccination with plasmids encoding the Leishmania major gp63 glycoprotein and CD40L results in a partial suppression of the inflammatory reaction after experimental infection. European Journal of Inflammation 2(2), 91-96.

Athanassopoulou, F., E. Karagouni, E. Dotsika, V. Ragias, J. Tavla, P. Christofilloyanis and I. Vatsos (2004). Efficacy and toxicity of orally administrated anti-coccidial drugs for innovative treatments of Myxobolus sp. infection in Puntazzo puntazzo.Diseases of Aquatic Organisms 62, 217-226.

Athanassopoulou, F., E. Karagouni, E. Dotsika, V. Ragias, J. Tavla and P. Christofilloyanis (2004). Efficacy and toxicity of orally administrated anticoccidian drugs for innovative treatments of Polysporoplasma sparis (Sitja-Bobadilla & Alvarez-Pellitero 1885) infection in Sparus aurata L. Journal of Applied Ichthyology 20, 345-354.

Tsagozis, P., E. Karagouni and E. Dotsika (2004). Dendritic cells pulsed with peptides of gp63 induce differential protection against experimental cutaneous leishmaniasis. International Journal of Immunopathology & Pharmacology 17(3), 343-352.

Papamattheou, M.G., J.G. Routsias, E.E. Karagouni, C. Sakarellos, M. Sakarellos-Daitsiotis, H.M. Moutsopoulos, A.G. Tzioufas and E.N. Dotsika (2004). T cell help is required to induce idiotypic-anti-idiotypic autoantibody network after immunization with complementary epitope 289-308aa of La/SSB autoantigen in non-autoimmune mice. Clinical & Experimental Immunology 135, 416-426.

Frydas, S., M. Hatzistilianou, E. Karagouni, B. Madhappan, N. D’Orazio, G. Riccioni, F. Conti, G. Carratelli and D. Kempuraj (2003). Chemokines and parasites. International Journal of Immunopathology & Pharmacology 16(3), 221-224.

Tsagozis, P., E. Karagouni and E. Dotsika (2003).CD8+ T cells with parasite-specific cytotoxic activity and the TC1 profile of cytokine and chemokine secretion develop in experimental visceral leishmaniasis. Parasite Immunology 25, 569-579.

2000-2009
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Sideris, V., G. Papadopoulou, E. Dotsika and E. Karagouni (1999). Asymtomatic canine leishmaniasis in greater Athens area, Greece.European Journal of Epidemiology 15, 271-276.

Frydas, S., M. Reale, D. Vacalis, R.C. Barbacane, F.C. Placido, I. Cataldo, M. Gioacchino, E. Karagouni, E. Dotsika, G. Anogiannakis, A. Trakatellis and P. Conti (1999). IgG, IgG1 and IgM response in Trichinella spiralis-infected mice treated with 4-deoxypirydoxine or fed a Vitamin B6-deficient diet. Molecular & Cellular Biochemistry 194(1-2), 47-52.

Eliades, P., E. Karagouni, I. Stergiatou and K. Miras (1998). A simple method for the serodiagnosis of human hydatid disease based on a protein A/colloidal dye conjugate. Journal of Immunological Methods 218, 123-132.

Doukas, V., F. Athanasopoulou, E. Karagouni and E. Dotsika (1998). Aeromonashydrophila infection in cultured see bass, Dicentrarchus labrax L. and Puntazzo puntazzo Cuvier from the Aegean Sea. Journal of Fish Diseases 21, 317-320.

Karagouni, E., A. Chryssikopoulos, T. Mantzavinos, N. Kanakas and E. Dotsika (1998). Ιnterleukin-1 and interleukin-1 may affect the implantation rate of patients undergoing in vitro fertilization-embryo transfer. Fertility & Sterility 70, 553-559.

Papadopoulou, G., E. Karagouni and E. Dotsika (1998). ISCOMs vaccine against experimental Leishmaniasis. Vaccine 16, 885-892.

Dotsika, E., E. Karagouni, B. Sundquist, B. Morein, A. Morgan, and M. Villacres-Eriksson (1997). Ιnfluence of Quillajasaponaria triterpenoid content on the immunomodulatory capacity of Epstein-Barr virus iscoms. Scandinavian Journal of Immunology 45, 261-268.

Chryssikopoulos A., T. Μantzavinos, N. Kanakas, E. Karagouni, E. Dotsika, and P.A. Zourlas (1996). Correlation of serum and follicular fluid concentrations of placental protein 14 and CA-125 in in vitro fertilization-embryo transfer patients. Fertility & Sterility 66(4), 599-603.

Sideris, V., E. Karagouni, G. Papadopoulou, A. Garyfallou and E. Dotsika (1996). Canine visceral leishmaniasis in the great Athens area, Greece. Parasite 3, 125-130.

Frydas, S., E. Karagouni, E. Dotsika, M. Reale, R.C. Barbacane, I. Vlemmas, G. Anogianakis, A. Trakatellis, and P. Conti (1996). Generation of TNF, IFN-, IL-6, IL-4 and IL-10 in mouse serum from trichinellosis: effect of the anti-inflammatory compound 4-deoxypyridoxine (4-DPD). Immunology Letters 49, 179-184. IF: 2,526

Haralabidis, S., E. Karagouni, S. Frydas, and E. Dotsika (1995). Immunoglobulin and cytokine profile in murine secondary hydatidosis. Parasite Immunology 17, 625-630.

Karagouni, E., S. Frydas, E. Dotsika, C. Himonas, P. Conti and A. Trakatellis (1995). Inhibition of TNF- and IL-6, but not IFN-, by 4-deoxypyridoxine (4-DPD) in Trichinella-infected mouse serum. International Journal of Immunopathology & Pharmacology 8(1), 9-14.

Fridas, S., A. Trakatellis, E. Karagouni, E. Dotsika, C. Himonas and P. Conti (1994). 4-Deoxypyridoxine inhibits chronic granuloma formation induced by potassium permanganate in vivo. Molecular & Cellular Biochemistry 136, 59-63.

Karagouni, E.E., E.N. Dotsika and A. Sklavounou (1994). Alteration in peripheral blood mononuclear cell function and serum cytokines in oral lichen planus. Journal of Oral Pathology & Medicine 23, 28-35.

Haralabidis, S., S. Frydas, E. Karagouni and C. Himonas (1992). Trichinella spiralis-Ab3 (IgG, IgM, IgA), IL-6 and TNF levels in murine trichinellosis. International Journal of Immunopathology & Pharmacology 5(3), 173-183.

Karagouni, E.E. and L. Kourounakis (1991). Anti-inflammatory and immunomodulating effects of the novel agent -(2-aminoethylamino)-2-butyrothienone. 3rd communication: Effect on suppressor cells and cytokines production in normal and adjuvant arthritic rats. Arzneimittel-Forschung/Drug Research, 41(I), 6, 647-651.

Kourounakis, L., A. Gavalas, E.E. Karagouni, D. Litina and P. Kourounakis (1991). Anti-inflammatory and immunomodulating effects of the novel agent -(2-aminoethylamino)-2-butyrothienone (-ABT). 2nd communication: Inhibitory effect on rat adjuvant induced disease. Arzneimittel-Forschung/Drug Research 41(I), 5, 537-541.

Karagouni, E.E. and L. Hadjipetrou-Kourounakis (1990). Regulation of isotype immunoglobulin and interleukin production by adjuvants in vitro. Journal of Clinical & Laboratory Immunology 33, 29-39.

Karagouni, E.E. and L. Hadjipetrou-Kourounakis (1990). Regulation of isotype immunoglobulin production by adjuvants in vivo. Scandinavian Journal of Immunology 31, 745-754.

Karagouni, E.E. and L. Hadjipetrou-Kourounakis (1990). Interleukins counteract opioid agonists immunosuppression. International Journal of Neuroscience 54(1-2), 157-163.

1990-1999
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Hadjipetrou-Kourounakis, L., E. Karagounis, E. Rekka and P. Kourounakis (1989). Immunosuppression by a novel analgesic-opioid agonist. Scandinavian Journal of Immunology 29, 449-458.

1989

MEMBERS

Evdokia Karagouni

Research Director
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Maria Agallou

Biologist, PhD, Post-doctoral Fellow (2011-today)
PhD Student: 2003-2010
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Maritsa Margaroni

Biologist, MSc, PhD
Post-doctoral Fellow: 2017, PhD Student: 2008-2016, MSc Student: 2005-2006
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Evita Athanasiou

Biologist, MSc, PhD
Post-doctoral Fellow: 2016-2017, PhD Student: 2012-2015, MSc Student: 2008-2009
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Dimitra Toubanaki

Chemist, PhD,
Post-doctoral Fellow: 2009-2010 and 2012-2015
Research Assosiate: 2016-2017
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Nikos Dogas

Medical laboratory technician, Biologist, MSc Student

Maria Agallou

Biologist (ΙΔΟΧ- ΝΙΑΡΧΟΣ),
Laboratory of Cellular Immunology (Dr. Karagouni)9
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Όνομα Ερευνητή

Ιδιότητα – Επάγγελμα
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Former Members (LINKS)

MSc Students
Eleni Pantazi (2015-2016)
Elisavet Tsiftsaki (2013-2015)
Sofia Nikolopoulou (2012-2013)
Golfo Voukelatou (2011-2012)

Undergraduate Students
Athina Vasilaki (2014-2016)
Sofia Ralli (2013-2014)

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