Light Microscopy Unit & FACS-Facility
LIGHT MICROSCOPY UNIT
The Light Microscopy Unit (LMU) of the HPI is one of the most modern and well-equipped Imaging Units in Greece and a member of the Greek Bioimaging-GR Consortium and the European Light Microscopy Initiative (ELMI- www.embl.org/elmi/). LMU is an open-access platform aiming to provide advanced Light microscopy and image analysis services to HPI, national or international academic scientists.
Current equipment in the Light Microscopy facility includes:
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New users of the Light Microscopy Unit (LMU) of HPI, as well as current users, who are starting a new project, should submit a project submission form at the Light Microscopy Unit of HPI. We need all necessary information to organize on time introductory sessions for using the instruments, as well as for teaching and supporting sample preparation techniques and new imaging protocols. You can download the LMU Project submission form, fill it in and send it to email@example.com.
Τraining in the use of microscopes and in image analysis applications
Training in the use of the microscopes and the image analysis software are conducted by the members of the Unit. Guides in the use of the Leica TCS-SP Confocal microscope and the wide-field Olympus IX-81 Cell-R imaging system can be found in the greek version of the website.
Image processing and analysis
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In the HPI-LMU, since its founding (2001), its users carried out projects that gave rise to a 39 publications in which members of the HPI LMU are listed as authors and/or the contribution of the HPI-LMU personnel is acknowledged.
The Scientists supporting the HPI-LMU have participated either as members or as Scientific responsibles in several grant applications succeeding to attract funding for the Unit either for organizing courses or for upgrading the equipment and development of new methodology from the following grants:
The HPI FACS-Facility is supported by a researcher and a part time technician who provide scientific consultation and technical assistance to the users. The instruments in the FACS-Facility are used by researchers of the Institute performing multiple studies, including the separation of cells (cell sorting), and the detection of several fluorescent and forward and side scatter parameters (FSC & SSC) that can be used in several applications including the characterization of immune cells and other cell populations, the detection of apoptosis, cell death, cell cycle analysis, cell division etc.