Study of a New Enzyme from the parasite Leishmania donovani with potential to become a new anti-leishmaniasis drug target

Scientists from the Hellenic Pasteur Institute EIP, members of the Laboratory “Intracellular Parasitism”, in collaboration with researchers from the “Genome Biology” unit at the European Laboratory of Molecular Biology (EMBL) in Germany and the “Biomechanics of Host Parasite Interactions” Research Group at the Advanced BioSciences Institute in the University of Grenoble Alpes, studied for the first time the expression of the protein LdTyrPIP_22 in Leishmania donovani parasites, giving results for its enzymatic activity as phosphatase, its subcellular localization, as well as its localization change within the parasite in conditions resembling those of the mammalian macrophage host.

Authors: Papadaki, Amalia, Olympia Tziouvara, Anastasia Kotopouli, Petrina Koumarianou, Anargyros Doukas, Pablo Rios, Isabelle Tardieux, Maja Köhn, and Haralabia Boleti.

“The Leishmania donovani LDBPK_220120. 1 Gene Encodes for an Atypical Dual Specificity Lipid-Like Phosphatase Expressed in Promastigotes and Amastigotes; Substrate Specificity, Intracellular Localizations, and Putative Role (s).” Frontiers in Cellular and Infection Microbiology 11 (2021): 85. (Impact Factor 4.123)

https://www.frontiersin.org/articles/10.3389/fcimb.2021.591868/full