Χαραλαμπία Μπολέτη

Haralabia Boleti | Intracellular Parasitism

Principal Investigator
+ 30 210-6478879
.img@.img CV

RESEARCH INTERESTS

My main interests lie in the research of pathogen-host cell interactions using molecular cellular biology and modern light microscopy imaging techniques.

Research in the “Intracellular parasitism group”, which I lead, focuses on aspects of the Molecular Cellular Biology of the flagellated protozoan parasite Leishmania donovani (L. donovani), and its interaction with the macrophages of the mammalian host. Aim of this research work is to contribute to the understanding of the molecular mechanisms underlying the initial stages of this parasitic infection as well as to identify and characterize new potential  molecular targets for the development of new anti-leismanial drugs

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The single celled eukaryotic protozoan parasite Leishmania with more than 20 species described belongs to the Trypanosomatidae order of the kinetoplastid class of protists. Parasites of the Leishmania genus are dimorphic, living as flagellated forms (promastigotes) in the gut of their sandfly vector and as aflagellated forms (amastigotes) mainly inside phagolysosomes of mammalian host phagocytes. Leishmania promastigotes (metacyclics) are transmitted to the mammalian host upon the blood meal of the insect vector (sandflies of the genus Phlebotomus or Lutzomyia).The first step of Leishmania interaction with the mammalian host, after the sand fly bite, is the entry into the phagocytic cells (neutrophils, macrophages, and dendritic) of the host immune system by phagocytosis. Macrophages are the cells in which the parasite establishes infection and by which it is carried in organs distant from the site of infection as the liver, spleen and bone marrow in the case of visceral Leishmaniasis. Survival and propagation of the parasite in the professional phagocytes is due to interference and subversion of host signaling pathways related to many aspects of cell physiology including the ones of phagolysosome biogenesis and apoptosis. Infection of mammals with anthroponotic or anthropozoonotic Leishmania species causes a wide spectrum of human cutaneous (CL) and visceral (VL) diseases known as Leishmaniases. An estimated 300–500,000 cases of VL, the most severe form of the disease which is fatal if not treated, and over 20,000 deaths occur annually. Although several drugs against the different forms of Leishmaniasis are available, there is an urgent need to develop new ones due to severe side effects and high cost of the existing chemotherapeutics and the development of drug resistant parasite strains. It is worth mentioning that no efficient vaccine against the different forms of Leismaniases exists.

RESEARCH PROGRAMS

Study of molecular mechanisms of Leishmania donovani (L. donovani) establishment of infection in professional phagocytes with focus on the role of parasite surface (i.e ecto-enzymes) or secreted molecules in the establishment of parasite life in the modified Leishmaniophorous phagolysosome.

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With advanced microscopy methods we image the spatio-temporal distribution of phosphoinositides (PIs) and other molecular markers of phagosome maturation on the membrane of the Leishmaniophorous phagosome from the moment of promastigote engulfment up to 4 hrs  after the initiation of parasite phagocytosis (Papadaki Α. PhD thesis 2015).

In parallel, we focus on studies of surface (ecto-phosphatases) or secreted parasitic molecules with aim to understand their possible role in the biogenesis of the Leishmaniophorous phagolysosome and the survival of Leishmania cells in it. We recently published a study on the characterization of an L. donovani membrane bound acid phosphatase (LdMAcP) that is localized at the external surface of the paraite’s plasma membrane and contributes to the parasitic virulence (Papadaki A. et al., Boleti H. 2015).

As secreted L. donovani molecules we study the structures and role of a protein from the family of sorting nexins discovered in a proteomics analysis of the promastigote secretome (Silverman JM et al. 2008) (Kourounis et al 2016, poster in the 67th conference of the HSBMB).

PUBLICATIONS

THESES

  • PhD, Amalia Papadaki, Medical School University of Ioannina, Defended on April 27, 2015
    Title: Study of the molecular mechanisms underlying Leishmania spp. survival within the phagocytes of the mammalian host (PDF)
  • MSc thesis, Drosos Kourounis, University of Athens, MSc Program «Applications of Biology in Medicine”. March 2017
    Title: Cloning and molecular characterization of the secreted nexin LdPIΒPnex with a PI binding domain from the protozoan parasite Leishmania donovani (PDF)
  • MSc Alexis GACHET-IEREMIADIS, M1 Master 1 Health engineering / Management of biotechnology, Grenoble Alpes University (UGA)  2016/17. defended in June 2017
    Title: Expression in E.coli and purification of a Leishmania donovani phosphoinositide binding protein from the nexin family (LdPIBPnex)
    (PDF)

CONFERENCE PRESENTATIONS

  • 67o Panhellenic conference of the Hellenic Society of Biochemistry and Molecular Biology, Ioannina, (25-27 Nov. 2017) Poster (prize)TitleLdPIΒPnex, a secreted nexin-like protein from the protozoan parasite Leishmania donovani. AuthorsDrosos Kourounis, Amalia Papadaki, Olympia Tziouvara, Haralabia Boleti (Poster)
  • Scientific Symposium of the Institut Pasteur International Network. Paris, France (10-13 Sept. 2014). Poster Title: The Leishmania membrane bound Histidine Acid Phosphatases (HAcPs) as putative virulence factors; Characterization of the LdMAcP, a Histidine Acid ecto-phosphatase from donovani. AuthorsΑmalia Papadaki, Ηaralabia Boleti (PDF)
  • Scientific International Meeting of Young Researchers from RIIP, Institut Pasteur, Paris, France (10 Nov. 2011). Commented Poster (oral presentation). Title: Phosphoinositide involvement in Leishmania donovani phagocytosis by Raw 264,7 macrophages. AuthorsΑmalia Papadaki, Ηaralabia Boleti (PDF)

Development of advanced microscopy protocols for imaging (in vitro or in vivo) intracellular pathogens with the use of digital image/video processing/analysis software. In our studies we use genetically modified Leishmania strains expressing GFP or RFP fluorescent proteins and mammalian macrophage cell lines (mouse Raw264.7) expressing GFP or YFP hybrid polypeptides with PI binding domains (PX or PH domains) or hybrid fluorescent GTPases (i.e. Rab5, Rab7) molecular markers of  phagolysosome biogenesis (Papadaki A. PhD thesis 2015).

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THESES

  • PhD, Amalia Papadaki, Medical School University of Ioannina, Defended on April 27, 2015
    Title: Study of the molecular mechanisms underlying Leishmania spp. survival within the phagocytes of the mammalian host (PDF)
  • Diploma, Maria Kotini, Department of Biology, University of Athens, Defended on Nov. 2009
    Title: Construction and characterization of transgenic Leishmania donovani strains expressing the red fluorescent protein mRFP1and of anti- mRFP1 polyclonal Abs (PDF)
  • MSc Olivia Paraskevi Tziouvara, Biology Dept, U. of Athens (MSc program Applications of Biology in Medicine) defended in October 2017
    Title: Molecular and functional characterization of the Tyrosine and phosphoinositide phosphatase LdTyrPIP_22, from the protozoan parasite Leishmania donovani. (PDF)

CONFERENCE PRESENTATIONS

  • Scientific International Meeting of Young Researchers from RIIP, Institut Pasteur, Paris, France (10 Nov. 2011). Commented Poster (oral presentation). Poster Title: Phosphoinositide involvement in Leishmania donovani phagocytosis by Raw 264,7 macrophages. AuthorsΑmalia Papadaki, Ηaralabia Boleti (PDF)
  • 60th Panhellenic conference of the Hellenic Society of Biochemistry and Molecular Biology, Athens, 20-22 Νοv. 2009
    Poster Title: Generation of transgenic Leishmania donovani expressing the Red Fluorescent Protein mRFP1: a tool for in vivo imaging of the parasite-host interaction Authors: Maria Kotini, Amalia Papadaki, Despina Smirlis, Ketty Soteriadou, Giorgos Diallinas &, Haralabia Boleti (PDF)

Structural and functional characterization of specific L. donovani phosphatases as candidate anti-Leishmanial drug targets. Main focus of our work is the study of the LdMacP (LdAcP3.1_AF149839.1) Histidine Acid ecto-phosphatase from L. donovani (Shakarian, A.M., et al.2002, Papadaki et al. 2015), and of two members from a family of phosphoinositide phosphatases expressed only in microorganisms, homologs of the  MptpB virulence factor from Mycobacterium tuberculosis (Beresford, N.J. et al. 2010). The two Ldonovani phosphatases under study are encoded by the Open Reading Frames (ORF) LDBPK_220120 and LDBPK_332990.

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PUBLICATIONS

  • Papadaki A, Politou AS, Smirlis D, Kotini MP, Kourou K, Papamarcaki T, Boleti H. (2015). The Leishmania donovani histidine acid ecto-phosphatase LdMAcP: insight into its structure and function. Biochem J. 467(3):473-86.

THESES

  • PhD, Amalia Papadaki, Medical School University of Ioannina, Defended on April 27, 2015
    Title: Study of the molecular mechanisms underlying Leishmania spp. survival within the phagocytes of the mammalian host
  • MSc, Anastasia Kotopouli, ΕΚΠΑ, Chemistry Department, «Biochemistry» Defended in Feb. 2014
    Title: Cloning, molecular and functional characterization of the putative PI phosphatase, LdPIP_22, of the parasitic protozoan  Leishmania donovani (PDF)
  • ΜSc, Mathias Richard, Faculté de Sciences, U. of Montpellier, MSc BioPMBB-IMH, Defence in Jun. 2015
    Title: Molecular Biology of the protozoan parasites Leishmania donovani: Cloning of an L. infantum putative phosphatase into plasmid vectors for expression of His-tagged or mCherry-tagged protein in bacteria, mammalian and Leishmania cells. (PDF)

CONFERENCE PRESENTATIONS

  • 66th Panhellenic conference of the Hellenic Society of Biochemistry and Molecular Biology, Athens (11-13 Dec. 2015). Oral presentation
    Poster Title: Biochemical characterization and subcellular localization of the tyrosine and phosphoinositide dual specificity phosphatase LdPIP_22, a potential drug target from Leishmania donovaniAuthorsAmalia Papadaki, Anastasia Kotopouli, Anargyros Doukas, Pablos Rios, Olivia Tziouvara, Maja Köhn, Haralabia Boleti (PDF)
  • Cost Action CM0801, 4th Annual Meeting. Crete (Sept. 19-21 2012).
    Poster Title: Study of specific Leishmania Atypical Lipid Phosphatases (ALPs) as putative virulence factors for parasite survival in host phagocytes and as potential drug targets. AuthorsAnastasia Kotopouli, Amalia Papadaki, Aris Doukas , Konstantinos Papadakos , Dionyssios Sgouras, Ntia Galanopoulou, Haralabia Boleti. (PDF)
  • 62ο Panhellenic conference of the Hellenic Society of Biochemistry and Molecular Biology, Athens (9-11 Dec. 2011).
    Poster Title: Study of specific Leishmania histidine acid phosphatases as putative virulence factors for parasite survival in host phagocytes and as potential drug targets. Authors: Papadaki Α., Smirlis D., Kourou, K., Politou A.S., Boleti Η. (PDF)

Validation of the Leishmania tarentolae (L. tarentolae) organism (non pathogenic to humans) as an alternative expression system for recombinant eukaryotic membrane proteins (Biotechnological applications) and as an in vitro system for the screening of anti-leismanial compounds.

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Validation of the Leishmania tarentolae (L. tarentolae) organism (non pathogenic to humans) as an alternative expression system for recombinant eukaryotic membrane proteins (Biotechnological applications) and as an in vitro system for the screening of anti-leismanial compounds.

We use the L.tarentolae promastigote cells for a) expression of mammalian nucleobase transporters (ΕVOTRAΝS project; b) expression of the human PRL3 phosphatase (IKYDA program, collaboration with the lab of Μaja Köhn EMBL, Heidelberg) and c) the production of L. donovani phosphatases for structural and functional analysis.

For the screening of candidate anti-leismanial drugs we have generated genetically modified L. tarentolae strains that express the red fluorescent protein mcherry with which, according to published protocols our group is open for collaboration for the screening of compounds with antiparasitic properties.

THESES

  • MSc, Anargyros DOUKAS, Biology Departments, University of Athens, MSc program in «Microbial BIotechnology». Defence in Jan. 2015
    Title: Construction and characterization of transgenic διαγονιδιακών Leishmania tarentolae strains for heterologous expression of the red fluorescent protein mCherry and of the nucleobase transporter rSNBT1 (from Rattus norvegicus) (PDF)

CONFERENCE PRESENTATIONS

  • 64th Panhellenic conference of the Hellenic Society of Biochemistry and Molecular Biology, Athens (6-9 Dec. 2013).
    Poster Title: Heterologous expression of the mammalian nucleobase transporter rSNBT1 in the LEXSY Leishmania tarentolae protein expression system. Authors Doukas Anargyros, Papakostas Konstantinos, Papadaki Amalia, Frillingos Stathis, Boleti Haralabia (PDF)

CURRENT GROUP MEMBERS

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Petrina Koumarianou

Biologist/Cell Biologist (ΒSc, MSc, PhD)
Post-doctoral fellow Sep. 2018
Email: .img@.img

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Olympia-Paraskevi Tziouvara

Biologist (BSc)
MSc Student (Applications of Biology to Medicine)
Sep. 2015- May 2017
PhD Student 2018-
Email: .img@.img

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Katerina Papakonstantinou

Pharmasist (BSc, MSc)
PhD Student,
Pharmacy School, Univesrity of Athens
Email: .img@.img

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Giorgos Dimotsantos

Biologist/Biotechnologist (BSc)
MSc student, Sep. 2018-
Βiology Department “Microbial Biotechnology”, University of Athens
Email: .img@.img

Μαρίνα Πετσανά95

Marina Petsana

Final year undergraduate student
Dep. of Informatics with applications in  Biomedicine,
University of Thessaly, Greece
July-August 2020

Danniel Carrasco

Daniel Carrasco
Molecular Biologist (BSc, MSc)
PhD student (Calmette Yersin PhD fellow) , Oct.1, 2020 –
University of Alcala, Madrid
Email: .img@.img

hakan sukur

Ηakan Nizam
5th year undergraduate student,
Biotechnology Department, Agricultural University of Athens
Practical training, Oct 2020.-January. 2021

OLDER GROUP MEMBERS / COLLABORATORS

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Panagiotis Samboutzis

Biologist, U. of Patras, Department of Environmental and Natural Resources Management
PhD Fellow (Collaborator from Dr Bourtzis’ lab) 2007-2008

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Maria Parakevi Kotini

Biologist Diploma thesis, Μarch 2008-Νοv. 2009

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Anastasia Kotopouli

ChemistΜSc Student (Biochemistry), Sep. 2012 – Jan. 2014

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Dr Rositsa Tropcheva

Food Biotechnologist (PhD)
Assistant Professor, “St Kliment Ohridski” University of Sofia, Bulgaria, Research Fellow, June 2014- July 2014 (Institut Pasteur International Network Chalmette Yersin fellowship)

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Anargyros Doukas

Chemist
Technical Assistant & ΜSc Student (Microbial Biotechnology), June 2012- Jan. 2015

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Mathias Richard

Faculté de Sciences, U. of Montpellier,
MSc Student – BioPMBB-IMH, Feb. 2015- Jul. 2015

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Amalia Papadaki

Veterinarian, ΜSc,
PhD Fellow, Jun 2009- May 2015
Post doctoral Fellow, Jun 2015-Jan. 2016

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Drosos Koyrounis

Biologist (BSc)
MSc Student (Applications of Biology to Medicine),
Sep. 2015-Mar. 2017

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Alexis Gachet-Ieremiadis

ΜSc Internship, Biotechnologist (BSc)
April 2017- June 2017

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Maria Boulougari

4rth year undergraduate student,
Biotechnology Dep., Agricultural U. of Athens
Summer student training, Jul. – Sep. 2019

SELECTED PUBLICATIONS

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Papadaki A. and Boleti H. (2019). Measurement of Acid Ecto-phosphatase Activity in Live Leishmania donovani Parasites. BioProtocols (A peer-reviewed protocol journal. No publication fee; no access fee) following journals’ invitation. Vol 9 (19). DOI:10.21769/BioProtoc.3384

Doukas A, Karena E, Botou M, Papakostas K, Papadaki A, Tziouvara O, Xingi E, Frillingos S, Boleti H. (2019). Heterologous expression of the mammalian sodium-nucleobase transporter rSNBT1 in Leishmania tarentolae. Biochim Biophys Acta Biomembr. 1861(9):1546-1557.

Braliou GG, Kontou PI, Boleti H, Bagos PG. (2019). Susceptibility to leishmaniasis is affected by host SLC11A1 gene polymorphisms: a systematic review and meta-analysis. Parasitol Res. 118(8):2329-2342. Review.

Frakolaki E, Kalliampakou KI, Kaimou P, Moraiti M, Kolaitis N, Boleti H, Koskinas J, Vassilacopoulou D, Vassilaki N. (2019). Emerging Role of l-Dopa Decarboxylase in Flaviviridae Virus Infections. Cells 8(8). ( PDF )

2019
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Papadaki A, Politou AS, Smirlis D, Kotini MP, Kourou K, Papamarcaki T, Boleti H. (2015). The Leishmania donovani histidine acid ecto-phosphatase LdMAcP: insight into its structure and function. Biochem J. 467(3):473-86. ( PDF )

2015
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Athanasopoulos A, Boleti H, Scazzocchio C, Sophianopoulou V. (2013). Eisosome distribution and localization in the meiotic progeny of Aspergillus nidulans. Fungal Genet Biol. 53:84-96. ( PDF )

2013
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Boleti H., Smirlis D., Dalagiorgou G., Meurs E., Christoforidis S., & Mavromara.P. (2010). ER targeting and retention of the HCV NS4B protein relies on the concerted action of multiple structural features including its transmembrane domains. Mol. Memb. Biol. 27(1):50-74. ( PDF )

2010
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Smirlis D, Boleti H, Gaitanou M, Soto M, Soteriadou K. (2009). Leishmania donovani RAN-GTPase interacts at the nuclear rim with linker histone H1. Biochem J. 10;424(3):367-74. ( PDF )

2009
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Vassilaki N, Boleti H, Mavromara P.(2008).Expression studies of the HCV-1a core+1 open reading frame in mammalian cells. Virus Res. 133(2):123-35. ( PDF )

2008
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Vassilakι Ν, Boleti Η. and Mavromara P. (2007). Expression studies of the core+1 protein of the hepatitis C virus 1a in mammalian cells: the influence of the core protein and proteasomes on the intracellular levels of core+1. FEBS J. 274(16):4057-74. ( PDF )

2007
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Boleti H. & Robotis, J.F (2004). Viral Hepatitis. xPharm 1.0, edited by S.J. Enna dn David, B. Bylund, Published by, Inc. Elsevier. Reviews-Electronic publications ( PDF )

Boleti H. (2004). Chlamydia Infections. xPharm 1.0, edited by S.J. Enna dn David, B. Bylund, Published by, Inc. Elsevier. ( Link )

2004
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Ibrahim-Granet, O., Philippe,B., Boleti, H., Boisvieux-Ulrich, E., Prévost, MC., Grenet, D., Stern, M., and JP Latgé. (2003). Phagocytosis and intracellular fate of Aspergillus fumigatus conidia in alveolar macrophages. Infection and Immunity, 71(2):891-903. ( PDF )

2003
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Boleti, H., Ojcius, D. and Dautry-Varsat, A. (2000). Fluorescent labelling of intra-cellular bacteria in living host cells. J. Microbiological Methods, 1;40 (3): 265-274. ( PDF )

Poupel, O., Boleti H., Axisa, S., and Tardieux, I. (2000). Toxfilin, a novel actin binding protein from Toxoplasma gondii, sequesters actin monomers and caps actin filaments. J. Mol. Biol. of the Cell, 11(1): 355-68. ( PDF )

2000
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Boleti, H., Benmerah, A., Ojcius, D., Cerf-Bensussan,N., Dautry-Varsat, A. (1999). Chlamydia infection of epithelial cells expressing dynamin and Eps15 mutants:clathrin-independent entry into cells and dynamin-dependent productive growth. J. of Cell Science, 112:1487-1496. ( PDF )

1999

ΕDUCATION

PhD theses

Amalia Papadaki, Medical School, U. of Ioannina, defended in April 2015
Title: Study of the molecular mechanisms underlying Leishmania spp. survival within the phagocytes of the mammalian host (PDF)

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MSc theses

Anastasia Kotopouli, Chemistry Dep., U. of Athens (MSc Program Biochemistry), defended in February 2014
Title: Cloning, molecular and functional characterization of the putative phosphoinositide phosphatase LdPIP22 from the parasitic protozoan Leishmania donovani (PDF) 

Anargyros Doukas, Biology Dept, U. of Athens (MSc program Microbial Biotechnology), defended in January 2015
Title: Construction and characterization of transgenic strains of the protozoan Leishmania tarentolae for heterologous expression of the red fluorescent protein mCherry and nucleobase transporter rSNBT1 from Rattus norvegicus. (PDF) 

Mathias Richard, Faculté de Sciences, U. of Montpellier, MSc BioPMBB-IMH, defended in June 2015
Title: Molecular Biology of the protozoan parasites Leishmania donovani: Cloning of an L. infantum putative phosphatase into plasmid vectors for expression of His-tagged or mCherry-tagged protein in bacteria, mammalian and Leishmania cells. (PDF) 

Olivia Paraskevi Tziouvara, Biology Dept, U. of Athens (MSc program Applications of Biology in Medicine) to be defended at the end of 2016/beginning of 2017
Title: Molecular and functional characterization of the Tyrosine and phosphoinositide phosphatase LdTyrPIP_22, from the protozoan parasite Leishmania donovani. (PDF)

Drosos Kourounis, Biology Dept, U. of Athens (MSc program Applications of Biology in Medicine) to be defended at the end of 2016/beginning of 2017
Title: Cloning and molecular characterization of the secreted Phosphoinositide Binding nexin-like protein LdPIΒPnex from the protozoan Leishmania donovani (PDF)

MSc Alexis Gachet-Ieremiadis, M1 Master 1 Health engineering / Management of biotechnology, Grenoble Alpes University (UGA)  2016/17. defended in June 2017
Title: Expression in E.coli and purification of a Leishmania donovani phosphoinositide binding protein from the nexin family (LdPIBPnex)
 (
PDF)

Diploma thesis

Maria Kotini, Diploma thesis, Biology Dept, of Athens, Defended in Nov. 2009.
Title: Cosntruction and characterization of trasngenic parasites Leishmania donovani expressing the red fluorescent protein mRFP1and of anti-mRFP1 polyclonal antibody.

Teaching in graduate programs(2006-2016)

2018 – Lecture “ The protozoan flagellated parasite Leishmania as a model for expression of recombinant eukaryotic proteins and for the study of ciliogenesis”. MSc program “Microbial Biotechnology” Biology Department, University of Athens.

2011-2017-Lectures «Leishmania tarantolae, a eucaryotic cell system with important biotechnological applications». MSc program «MICROBIAL BIOTECHNOLOGY», Department of Biology, University of Athens

2015 – Lectures «Host-Cell mechanisms that become targets of intracellular pathogens» MSc program «APPLICATIONS OF BIOLOGY IN MEDICINE» Biology Department, University of Athens

2011-2014 – Lectures «Study of Cellular Organization with modern Fluorescene Microscopy techniques» MSc program “BIOCHEMISTRY”, Department of Chemistry, University of Athens

Organization of International and courses (2006-2016)

2007-2008

Cutting edge technologies and their contribution to the Biomedical Sciences
3-day practical courses for Biology Teachers of Secondary School education. An activity for diffusion of Advanced Scientific Knowledge to selected groups of society. ( Poster )

2013

Digital image processing/analysis tools in Light Microscopy: From the basics and beyond. Institut Pasteur Network  Regional course 2013 ( Poster )

2016

Cell Biology and infection: Digital Image Processing/Analysis Tools for Quantitative Light Microscopy Imaging. Institut Pasteur International Network course 2016 ( Poster )

FUNDING

2020

CALMETTE YERSIN PhD fellowship Institut Pasteur International Network (IPIN)
PhD fellowship to Daniel Carrasco Navarro
Project title: “ Identification of molecular partners and functional characterization of a secreted Sorting Nexin (SNX) Protein from the protozoan parasite L. donovani; the first SNX to be studied from Leishmania spp.”
Grant initiation date: October 2020; Grant duration: 36 months
Budget: 60,000€

2019

Ministry of Development and Investment/grant Code ΕΒΒΜ103.
“Supporting researchers with emphasis on young researchers – B cycle”-
“Human Resources Development, Education and Lifelong Learning” on Priority Axis 6, co-funded by the European Social Fund (ESF).
Project title: 
In vitro study of the anticancer properties of polyphenols from greek olive oil.
Grant initiation date: 
End of 2019 Grant duration: 15 months
Budget: 
45,500€

H.F.R.I (Hellenic Foundation for Research and Innovation)
PhD grant (Olympia Tziouvara)
Project title: “PI phosphatases and PI binding proteins of the Leishmania protozoan parasite: Characterization of two representative molecules and study of their role in the parasite life cycle and in the infection of the mammalian host”
Grant initiation date: October 2019; Grant duration: 30 months
Budget: 27,000€

2018

ΒΙΟΙΜAGING GR“Hellenic Research Infrastructure for the Imaging and Observation of Fundamental Processes in Biology and Medicine” (MIS 5002755) which is implemented under the Action “Reinforcement of the Research and Innovation Infrastructure”, funded by the Operational Programme “Competitiveness, Entrepreneurship and Innovation” (NSRF 2014-2020) and co-financed by Greece and the European Union (European Regional Development Fund). Responsible for 2WPs of the HPIs participation in the Infrastructure Network.

«INSPIRED: The National Research Infrastructure on Integrated Structural Biology, Drug Screening Efforts and Drug target functional characterization» MIS code 5002550 WP.2.1, WP 2.2

2017

KRIPIS II (2017-2019): DEVELOPMENT GRANTS FOR RESEARCH INSTITUTIONS/Greek General Secretariat for Research and Technology
Project title: “Infectious, autoimmune and neurodegenerative diseases: study of the pathogenetic mechanisms and development of diagnostic, prognostic and therapeutic approaches” Subproject ΠΕ 3.3: Structural and functional characterization of the Tyrosine and Phosphoinositide phosphatase LdTyrPIP_22 of the protozoan parasite Leishmania donovani (Μ4-Μ24) with aim to point out  new chemotherapeutical targets from antileismanial drugs (Coordinator Hellenic Pasteur Institute).
Budget for ‘Intracellular parasitism team22,900 €

2014

IKYDA (2014-2015) – PROGRAM of PROMOTION OF EXCHANGE AND SCIENTIFIC COOPERATION GREECE – GERMANY. Project title: LeishPhospho: Molecular characterization of specific phosphatases from the intracellular protozoan parasite Leishmania donovani and exploration of their potential as drug targets. Scientific Responsible of Greek team: Dr Haralabia Boleti, Scientific Responsible of the German teamDr Μaja Köhn
Budget:10,000 €

Sponsorship by Abbvie Pharmaceutical to the research team of Intracellular parasitism for research on Leishmaniasis. Scientific Responsible: Dr Haralabia Boleti. Budget: 5,000 €

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2013

PLATON-BILATERAL S & T COOPERATION GREECE –FRANCE (2013-2015).
Project title: LeishPhosphoToxMolecular and functional characterization of specific phosphatases from the anthropozoonotic intracellular protozoan parasites Leishmania donovani and Toxoplasma gondii and investigation of their role in the parasite interaction with host cells; Exploration of their potential as drug targets.
Scientific Responsible of Greek team: Dr Haralabia Boleti,
Scientific Responsible of  French teamDr Isabelle Tardieux
Budget: 30,000 €

ΚΡΗΠΙΣ Ι (2013-2015): GREEK GENERAL SECREATARIAT of RESEARCH and TECHNOLOGY
Project title: Infectious and Neurodegenerative diseases in the 21st century. From the study of basic mechanisms to development of  translational research and cutting edge technologies with aim to improve preventive diagnosis  and treatment. Subproject EE2.5: Survival mechanisms of the L. donovani parasites in the macrophage host cell and identification of virulence factors.  Subproject EE 4.5:  Identification, evaluation and characterization of candidate molecular targets for the development of anti-leismanial drugs. (coordinations: Hellenic Pasteur Insitute).
Budget for Intracellular parasitism group: 42,900 €

2012

THALES (2012-2015): GREEK MINISTRY OF EDUCATION
Project title: EVOTRANS-Membrane Transport: Sturcture-Function relationships and Evolution.  Project coordiantor: E. Frilliggos. University of Ioannina. Subproject Π.Ε.1-4 scientific responsible: H. Boleti
Budget for Intracellular Parasitism group: 40,000 €

2011

Sponsorship by the pharmaceutical company Genesis PharmaProject titel: Study of the molecular mechanisms by which the protozoan parasite Leishmania donovani infects humans and domestic animals to cause the disease of Kala azar. Scientific responsible: Dr Haralabia Boleti
Budget: 15.000 €

HONORS

2016

Poster award at the 67ο Panhellenic conference of the Hellenic Society of Biochemistry and Molecular Biology, Ioannina, (25-27 Nov. 2017)Title: LdPIΒPnex, a secreted nexin-like protein from the protozoan parasite Leishmania donovani. AuthorsDrosos Kourounis, Amalia Papadaki, Olympia Tziouvara, Haralabia Boleti, Intracellular parasitism group, Hellenic Pasteur Institute

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