Laboratory of Medical Microbiology


Dionyssios Sgouras | Department of Microbiology
Research Director
Contact:
210-6478824 | @
| CV
ABOUT
Research in the LMM is centered on mechanisms by which mainly bacterial, but also viral pathogens can manifest their pathogenic role. The model organism studied is the human gastric pathogen Helicobacter pylori, which has been recognized as the etiologic factor for the development of chronic gastritis and peptic ulcer and has been classified as the primary risk factor for the development of gastric adenocarcinoma (class 1 carcinogen, by WHO). LMM functions as a National Reference Laboratory for Helicobacter infections, since present activities fully extend to diagnosis of the infection, laboratory surveillance of antimicrobial susceptibility of clinical isolates, full virulence factor characterization in H. pylori isolates, phylogenetic analysis of clinical isolates, based on specific gene or whole genome sequencing and preservation of a complete Biobank of clinical strains. LMM staff has participated in the team of microbiology experts set by the Hellenic Society of Gastroenterology, for the preparation of the consensus statement on the diagnosis, clinical management and treatment of H. pylori infection, in Greece (Georgopoulos et al., Hellenic consensus on Helicobacter pylori infection. Ann Gastroenterol. 2020)
RESEARCH PROGRAMS
FUNDING
PUBLICATIONS
Diechler S, Chichirau BE, Posselt G, Sgouras DN, Wessler S. (2021). Helicobacter pylori CagA EPIYA Motif Variations Affect Metabolic Activity in B Cells. Toxins (Basel); 13(9):592. doi: 10.3390/toxins13090592.
Bousali, M., Dimadi, A., Kostaki, E.-G., Tsiodras, S., Nikolopoulos, G.K., Sgouras, D.N., Magiorkinis, G., Papatheodoridis, G., Pogka, V., Lourida, G., Argyraki, A., Angelakis, E., Sourvinos, G., Beloukas, A., Paraskevis, D., Karamitros, T. (2021) SARS‐CoV‐2 molecular transmission clusters and containment measures in ten European regions during the first pandemic wave. Life; 11 (3), 219, doi: 10.3390/life11030219.
Saliba L., Zoumpopoulou G., Anastasiou R., Hassoun G., Karayiannis Y, Sgouras D., Tsakalidou E., Deiana P., Montanari L. and Mangia N.P. (2021) Probiotic and safety assessment of Lactobacillus strains isolated from Lebanese Baladi goat milk International Dairy Journal; 120, 105092.
Kontizas, E. and D. N. Sgouras. “Review – Pathogenesis of Helicobacter pylori infection” (2021) Microb Health Dis; 3: e539 part of the Special Issue: The Year in Helicobacter 2021, 34th International Work-shop on Helicobacter & Microbiota in Inflammation & Cancer Virtual Conference, 12 September 2021
Kontizas, E.; Tastsoglou, S.; Karamitros, T.; Karayiannis, Y.; Kollia, P.; Hatzigeorgiou, A.G.; Sgouras, D.N. (2020). Impact of Helicobacter pylori Infection and Its Major Virulence Factor CagA on DNA Damage Repair Microorganisms; 8(12), 2007.
2020Mangia, N.P.; Saliba, L.; Zoumpopoulou, G.; Chessa, S.; Anastasiou, R.; Karayiannis, Ι.; Sgouras, D.; Tsakalidou, E.; Nudda, A. (2019) Goat Milk with Different Alpha-s1 Casein Genotype (CSN1S1) Fer-mented by Selected Lactobacillus paracasei as Potential Functional Food. Fermentation; 5, 55. https://doi.org/10.3390/fermentation5030055
2019Georgopoulos SD, Papastergiou V, Martinez-Gonzalez B, Xirouchakis E, Familias I, Sgouras D, Mentis A, Karatapanis S. (2018) Hybrid therapy as first-line regimen for Helicobacter pylori eradication in a high clarithromycin resistance area: a prospective open-label trial. Ann Gastroenterol. Mar-Apr; 31(2):205-210. doi: 10.20524/aog.2017.0221.
2018Bugaytsova J, Chernov YA, Gideonsson P, Henriksson S, Mendez M, Sjöström R, Mahdavi J, Shev-tsova A, Quintana-Hayashi M, Ilver D, Moskalenko R, Aisenbrey C, Bylund G, Schmidt A, Moonens K, Björnham O, Brännström K, Königer V, Vikström S, Rakhimova L, Ögren L, Hofer A, Liu H, Goldman M, Whitmire JM, Younson JS, Kelly CG, Gilman RH, Chowdhury A, Mukhopadhyay AK, Nair BG, Papadakos KS, Martinez-Gonzalez B, Sgouras DN, Engstrand L, Unemo M, Danielsson D, Suerbaum S, Oscarson S, Morozova-Roche L, Gröbner G, Holgersson J, Esberg A, Strömberg N, Eldridge A, Chromy BA, Hansen LM, Solnick JV, Schedin S, Lindén SK, Haas R, Dubois A, Merrell DS, Remaut H, Arnqvist A, Berg DE, Borén T. (2017) Adaptation of the oncopathogen H. pylori during chronic in-fection and to gastric disease by acid responsive adherence Cell Host & Microbe. Mar 8;21(3):376-389, doi: 10.1016/j.chom.2017.02.013
2017Georgopoulos SD, Xirouchakis E, Martinez-Gonzalez B, Zampeli E, Grivas E, Spiliadi C, Sotiropoulou M, Petraki K, Zografos K, Laoudi F, Sgouras D, Mentis A, Kasapidis P, Michopoulos S. (2016) Ran-domized clinical trial comparing ten day concomitant and sequential therapies for Helicobacter pylori eradication in a high clarithromycin resistance area. Eur J Intern Med. pii: S0953-6205(16)30080-2. doi: 10.1016/j.ejim.2016.04.011.
Costa AM, RM Ferreira, I Pinto-Ribeiro, IS Sougleri, MJ Oliveira, L Carreto, MA Santos, DN Sgouras, F Carneiro, M Leite and C Figueiredo (2016) Helicobacter pylori activates matrix metalloproteinase-10 in gastric epithelial cells via EGFR and ERK-mediated pathways. J Infect Dis. 213(11): 1767-76 doi: 10.1093/infdis/jiw031
Sougleri IS, KS Papadakos, MP Zadik, M Mavri-Vavagianni, AF Mentis and DN Sgouras (2015) Heli-cobacter pylori CagA protein induces factors involved in the Epithelial-to–Mesenchymal-Transition (EMT) in infected gastric epithelial cells in an EPIYA- phosphorylation-dependent manner. FEBS J. 283(2): 206-220 doi: 10.1111/febs.13592
Sgouras DN, Trang TT, Yamaoka Y. (2015) Pathogenesis of Helicobacter pylori Infection. Helicobac-ter. Sep;20 Suppl 1:8-16. doi: 10.1111/hel.12251.
Tropcheva R, Hristova J, Georgieva R, Salageanu A, Sgouras DN, Danova S (2013) In vitro assess-ment of prebiotic utilization by dairy lactobacilli. Bulgarian J. Agric. Sci, 19, 105-107.
Georgopoulos SD, Xirouchakis E, Martinez-Gonzalez B, Sgouras DN, Spiliadi C, Mentis AF, and Laoudi F. (2013) Clinical evaluation of a ten day regimen with Esomeprazole, Metronidazole, Amoxicil-lin, and Clarithromycin for the eradication of Helicobacter pylori in a high Clarithromycin resistance ar-ea. Helicobacter. 2013 Dec; 18(6):459-67.
Papadakos KS, Sougleri IS, Mentis AF, Hatziloukas E, Sgouras DN. (2013) Presence of Terminal EPIYA Phosphorylation Motifs in Helicobacter pylori CagA Contributes to IL-8 Secretion, Irrespective of the Number of Repeats. PLoS One 8:e56291.
Papadakos KS, Sougleri IS, Mentis AF, Sgouras DN. (2013) A Mutagenesis Method for the Addition and Deletion of Highly Repetitive DNA Regions: The Paradigm of EPIYA Motifs in the cagA Gene of Helicobacter pylori. Helicobacter; 18(3):229-41.
Mueller D, Tegtmeyer N, Brandt S, Yamaoka Y, De Poire E, Sgouras D, Wessler S, Torres J, Smolka A, Backert S. (2012) c-Src and c-Abl kinases control hierarchic phosphorylation and function of the CagA effector protein in Western and East Asian Helicobacter pylori strains. Journal of Clinical Inves-tigation 122:1553-1566.
Altman E, Chandan V, Harrison BA, Panayotopoulou EG, Roma-Giannikou E, Li J, Sgouras DN. (2012) Helicobacter pylori isolates from Greek children express type 2 and type 1 Lewis and alpha 1,6-glucan antigens in conjunction with a functional type IV secretion system. Journal of Medical Mi-crobiology 61:559- 566.
Boziki M, Grigoriadis N, Deretzi G, Lagoudaki R, Panayotopoulou E, Sgouras D, Mentis A, Tascos N, Kountouras J. (2012) Helicobacter pylori Immunomodulative Properties in a Mouse Model of Multiple Sclerosis. Immunogastroenterology 1: 34-39
Breurec S, Michel R, Seck A, Brisse S, Come D, Dieye FB, Garin B, Huerre M, Mbengue M, Fall C, Sgouras DN, Thiberge JM, Dia D, Raymond J. (2011) Clinical relevance of cagA and vacA gene pol-ymorphisms in Helicobacter pylori isolates from Senegalese patients. Clinical Microbiology and Infec-tion 18:153-159.
Breurec S, Guillard B, Hem S, Papadakos KS, Brisse S, Huerre M, Monchy D, Oung C, Sgouras DN, Tan TS, Thiberge J-M, Vong S, Raymond J, Linz B. (2011) Expansion of European vacA and cagA alleles to East-Asian Helicobacter pylori strains in Cambodia. Infection Genetics and Evolution 11:1899-1905.
Roma-Giannikou E, Roubani A, Sgouras DN, Panayiotou J, van-Vliet C, Polyzos A, Roka K, Daikos G. (2010) Endoscopic Tests for the Diagnosis of Helicobacter pylori Infection in Children: Validation of Rapid Urease Test. Helicobacter 15:227-232.
Panayotopoulou EG, Sgouras DN, Papadakos KS, Petraki K, Breurec S, Michopoulos S, Mantzaris G, Papatheodoridis G, Mentis A, Archimandritis A. (2010) CagA and VacA Polymorphisms Are Associat-ed with Distinct Pathological Features in Helicobacter pylori-Infected Adults with Peptic Ulcer and Non-Peptic Ulcer Disease. Journal of Clinical Microbiology 48:2237-2239.
Sgouras DN, Panayotopoulou EG, Papadakos K, Martinez-Gonzalez B, Roumbani A, Panayiotou J, vanVliet-Constantinidou C, Mentis AF, Roma-Giannikou E. (2009) CagA and VacA Polymorphisms Do Not Correlate with Severity of Histopathological Lesions in Helicobacter pylori-Infected Greek Children. Journal of Clinical Microbiology 47:2426-2434.
Maragkoudakis PA, Papadelli M, Georgalaki M, Panayotopoulou EG, Martinez-Gonzalez B, Mentis AF, Petraki K, Sgouras DN, Tsakalidou E. (2009) In vitro and in vivo safety evaluation of the bacteri-ocin producer Streptococcus macedonicus ACA-DC 198. International Journal of Food Microbiology 133:141-147.
Alam MZ, Haralambous C, Kuhls K, Gouzelou E, Sgouras D, Soteriadou K, Schnur L, Pratlong F, Schoenian G. (2009) The paraphyletic composition of Leishmania donovani zymodeme MON-37 re-vealed by multilocus microsatellite typing. Microbes and Infection 11:707-715.
Paraschos S, Magiatis P, Mitakou S, Petraki K, Kalliaropoulos A, Maragkoudakis P, Mentis A, Sgour-as D, Skaltsounis A-L. (2007) In vitro and in vivo activities of chios mastic gum extracts and constitu-ents against Helicobacter pylori. Antimicrobial Agents and Chemotherapy 51:551-559.
Panayotopoulou EG, Sgouras DN, Papadakos K, Kalliaropoulos A, Papatheodoridis G, Mentis AF, Ar-chimandritis AJ. (2007) Strategy to characterize the number and type of repeating EPIYA phosphory-lation motifs in the carboxyl terminus of CagA protein in Helicobacter pylori clinical isolates. Journal of Clinical Microbiology 45:488-495.
Sgouras DN, Panayotopoulou EG, Martinez-Gonzalez B, Petraki K, Michopoulos S, Mentis A. (2005) Lactobacillus johnsonii La1 attenuates Helicobacter pylori-associated gastritis and reduces levels of proinflammatory chemokines in C57BL/6 mice. Clinical and Diagnostic Laboratory Immunology 12:1378- 1386.
2005Sgouras D, Maragkoudakis P, Petraki K, Martinez-Gonzalez B, Eriotou E, Michopoulos S, Kalantzopoulos G, Tsakalidou E, Mentis A. (2004) In vitro and in vivo inhibition of Helicobacter pylori by Lactobacillus casei strain Shirota. Applied and Environmental Microbiology 70:518-526.
De Vuyst L, Makras L, Avonts L, Holo H, Yi Q, Servin A, Fayol-Messaoudi D, Berger C, Zoumpopou-lou G, Tsakalidou E, Sgouras D, Martinez-Gonzales B, Panayotopoulou E, Mentis A, Smarandache D, Savu L, Thonart P, Nes I. (2004) Antimicrobial potential of probiotic or potentially probiotic lactic acid bacteria, the first results of the International European Research Project PROPATH of the PROEU-HEALTH cluster. Microbial Ecology in Health and Disease 16:125-130. Δείκτης Απήχησης: 0.780, Αναφορές: 16
Hynes SO, Broutet N, Wadstrom T, Mikelsaar M, O'Toole PW, Telford J, Engstrand L, Kamiya S, Mentis AF, Moran AP. Phenotypic variation of Helicobacter pylori isolates from geographically distinct regions detected by lectin typing. J Clin Microbiol. 2002 Jan;40(1):227-32.
2002Kalpoutzakis E, Aligiannis N, Mentis A, Mitaku S, Charvala C. Composition of the essential oil of two Nepeta species and in vitro evaluation of their activity against Helicobacter pylori. Planta Medica, 2001:67,880-3.
Petridou Ε, Dalamaga M, Mentis A, Skalkidou A, Moustaki M, Karpathios T, Trichopoulos D, and the Childhood Haematologists-Oncologists Group. Evidence supporting the infectious aetiology of child-hood leukaemia: the role of low herd immunity. Cancer Causes Control Journal, 2001:12,645-52.
Magiatis P, Spanakis D, Mitaku S, Tsitsa E, Mentis A, Harvala C. Verbalactone, a new macrocyclic dimer lactone from the roots of Verbascum undulatum with antibacterial activity. Nat Prod, 2001:64, 1093-4.
Georgopoulos, S. Ladas, S Karatapanis, AF Mentis, Ch Spiliadi, V. Artikis, SA Raptis. Factors that may affect treatment outcome of triple Helicobacter pylori eradication therapy with omeprazole, amoxicillin, and clarithromycin. Dig Dis Sci, 2000;45:63-67.
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