THUNDER Imager 3D Live Cell is designed for imaging cell culture assays. It allows a clear view of details within an intact sample, in real time without out-of-focus blur. This performance advantage is achieved with Computational Clearing opto-digital method. THUNDER Imager helps to maintain optimal physiological conditions by minimizing photobleaching, providing high-performance imaging and high-throughput of data leading to better workflow efficiency and statistics. The technical characteristics of the system are:
- Leica DMi8 inverted microscope
- pE-400max MB LLG light fluorescence (400, 450, 545, 635 nm excitation)
- Objectives: DRY: 5x, 10x, 20x, 40x, oil immersion: 63x
- Leica K8 Camera for fluorescence imaging
- Okolab environmental chamber with controlled temperature, humidity and CO2 concentration
- Applications:
- Imaging of fixed samples, 3D cell culture assays, stem cells, spheroids, organoids
- Live cell imaging
- Compatibility with multi-well carriers
- Removal of out-of-focus blur with computational clearing
The Leica Thunder Imager live cell has been acquired through National Recovery and Resilience Grant funding(MIS 5174647) and was installed in the HPI-BU in July 2025.